﻿89 
  

  

  neither 
  was 
  any 
  such 
  growth 
  detected 
  in 
  eggs 
  which 
  showed 
  no 
  evi- 
  

   dence 
  of 
  development. 
  The 
  notes 
  of 
  these 
  experiments 
  do 
  not 
  give 
  

   details 
  as 
  to 
  numbers 
  and 
  proportions, 
  but 
  these 
  were 
  ascertained 
  by 
  

   removing 
  one 
  hundred 
  and 
  forty 
  eggs 
  from 
  this 
  lot 
  May 
  22 
  and 
  

   keeping 
  them 
  under 
  identical 
  conditions 
  with 
  the 
  remainder. 
  These 
  

   began 
  to 
  hatch 
  May 
  29, 
  the 
  air 
  having 
  been 
  continuously 
  saturated 
  

   from 
  the 
  beginning, 
  and 
  by 
  the 
  30th 
  forty-seven 
  young 
  had 
  emerged; 
  

   by 
  the 
  31st 
  eighty-eight; 
  and 
  by 
  June 
  1, 
  one 
  hundred 
  and 
  four. 
  One 
  

   more 
  hatched 
  June 
  3, 
  and 
  the 
  remaining 
  thirty-five 
  did 
  not 
  hatch 
  

   at 
  all. 
  

  

  A 
  comparison 
  with 
  other 
  lots 
  would 
  indicate 
  that 
  this 
  treatment 
  

   of 
  the 
  eggs 
  may 
  have 
  had 
  the 
  effect 
  to 
  prevent 
  the 
  hatching 
  of 
  an 
  

   unusually 
  large 
  proportion, 
  and 
  to 
  drown 
  many 
  of 
  the 
  young 
  as 
  they 
  

   emerged. 
  

  

  Effed 
  of 
  Droidk 
  on 
  Hatching 
  of 
  ChinchMngs' 
  Eggs.— 
  One 
  

   hundred 
  and 
  forty 
  eggs 
  recently 
  laid 
  were 
  placed 
  May 
  9 
  on 
  dry 
  blot- 
  

   ting 
  paper 
  in 
  a 
  small 
  covered 
  glass 
  dish 
  and 
  kept 
  continuously 
  under 
  

   daily 
  observation 
  until 
  June 
  6. 
  Forty-three 
  of 
  these 
  eggs 
  hatched, 
  

   the 
  remainder 
  shrinking 
  and 
  shriveling 
  without 
  hatching. 
  None 
  of 
  

   the 
  young, 
  however, 
  succeeded 
  in 
  escaping 
  entirely 
  from 
  the 
  egg, 
  

   but 
  all 
  died 
  with 
  about 
  a 
  third 
  of 
  the 
  body 
  sticking 
  in 
  the 
  shell. 
  

  

  Effect 
  of 
  Exposing 
  Chinch-bugs' 
  Eggs 
  to 
  the 
  Fungus 
  of 
  White 
  

   Muscardine. 
  — 
  May 
  9, 
  1895, 
  a 
  large 
  number 
  of 
  chinch-bugs" 
  eggs 
  

   recently 
  hatched 
  were 
  placed 
  on 
  blotting 
  .paper 
  in 
  a 
  shallow 
  glass 
  

   dish 
  with 
  a 
  layer 
  of 
  damp 
  sand 
  in 
  the 
  bottom, 
  and 
  were 
  then 
  well 
  

   covered 
  with 
  spores 
  of 
  the 
  fungus 
  of 
  white 
  muscardine 
  (Sporotri- 
  

   chum) 
  from 
  adult 
  chinch-bugs 
  collected 
  May 
  7 
  in 
  the 
  field. 
  The 
  

   sand 
  and 
  blotting 
  paper 
  were 
  kept 
  constantly 
  moist 
  and 
  the 
  air 
  in 
  

   the 
  dish 
  thoroughly 
  saturated, 
  water 
  standing 
  in 
  drops 
  upon 
  the 
  

   under 
  surface 
  of 
  the 
  cover 
  the 
  greater 
  part 
  of 
  the 
  time. 
  Many 
  of 
  

   the 
  eggs 
  began 
  to 
  show 
  traces 
  of 
  development 
  by 
  the 
  20th 
  of 
  

   May, 
  but 
  a 
  few 
  remained 
  unchanged. 
  The 
  first 
  young 
  bug 
  appeared 
  

   May 
  22. 
  It 
  was 
  especially 
  treated 
  with 
  fungus 
  spores 
  placed 
  on 
  its 
  

   body 
  with 
  a 
  needle. 
  By 
  May 
  30 
  the 
  young 
  were 
  hatching 
  somewhat 
  

   freely 
  and 
  were 
  having 
  difficulty 
  in 
  crawling 
  up 
  the 
  very 
  moist 
  sur- 
  

   face 
  of 
  the 
  blotting 
  paper. 
  Several 
  of 
  them, 
  however, 
  had 
  estab- 
  

   lished 
  themselves 
  on 
  a 
  wheat 
  leaf 
  placed 
  in 
  the 
  dish 
  for 
  their 
  benefit. 
  

   Additional 
  spores 
  of 
  muscardine 
  fungus 
  were 
  introduced 
  at 
  this 
  time 
  

   from 
  another 
  source. 
  May 
  31 
  several 
  dead 
  bugs 
  were 
  seen, 
  recently 
  

   hatched 
  and 
  sticking 
  to 
  the 
  blotting 
  paper, 
  while 
  adults 
  were 
  crawl- 
  

   ing 
  about 
  and 
  some 
  were 
  feeding 
  on 
  the 
  wheat. 
  June 
  1 
  several 
  of 
  

   these 
  dead 
  bugs 
  were 
  crushed 
  on 
  a 
  slide 
  in 
  distilled 
  water 
  and 
  exam- 
  

   ined 
  microscopically. 
  After 
  having 
  been 
  thoroughly 
  and 
  repeatedly 
  

   washed 
  their 
  bodies 
  were 
  found 
  completely 
  filled 
  with 
  mycelial 
  

   threads, 
  evidently 
  those 
  of 
  Sporofrichum 
  glohiiJifo-iiui, 
  and 
  a 
  surface 
  

   growth 
  of 
  this 
  fungus 
  had 
  appeared 
  at 
  the 
  broken 
  end 
  of 
  a 
  mutilated 
  

   leg. 
  There 
  were 
  no 
  other 
  external 
  traces 
  of 
  this 
  fungous 
  infection 
  on 
  

   the 
  young 
  at 
  this 
  time. 
  A 
  microscopical 
  examination 
  of 
  eggs 
  ap- 
  

   parently 
  not 
  likely 
  to 
  hatch 
  was 
  made 
  June 
  3. 
  They 
  were 
  carefully 
  

   washed 
  in 
  distilled 
  water 
  and 
  crushed 
  on 
  a 
  glass 
  slide 
  and 
  examine'' 
  

  

  