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  microscopically. 
  No 
  bacterial 
  affection 
  was 
  detected, 
  but 
  mycelial 
  

   threads, 
  apparently 
  of 
  Sporotrichum 
  glohuliferurn, 
  were 
  present 
  in 
  

   them. 
  Bodies 
  of 
  young 
  bugs 
  were 
  likewise 
  examined 
  June 
  6 
  with 
  a 
  

   similar 
  result. 
  One 
  especially 
  signifisant 
  examination 
  was 
  made 
  of 
  an 
  

   egg 
  apparentl}^ 
  nearly 
  ready 
  to 
  hatch, 
  containing, 
  in 
  fact, 
  a 
  young 
  bug 
  

   quite 
  fully 
  devolopei. 
  The 
  egg 
  was 
  thoroughly 
  washed 
  three 
  times 
  

   in 
  distilled 
  water, 
  and 
  then 
  crushed 
  under 
  a 
  cover-glass. 
  There 
  were 
  

   mycelial 
  threads 
  in 
  the 
  body 
  cavity 
  of 
  the 
  bug, 
  in 
  practically 
  the 
  

   same 
  condition 
  as 
  those 
  found 
  in 
  dead 
  bugs 
  after 
  hatching. 
  

  

  August 
  6, 
  1895, 
  a 
  hundred 
  freshly 
  laid 
  eggs 
  were 
  placed 
  on 
  

   blotting 
  paper 
  over 
  damp 
  earth 
  in 
  a 
  covered 
  dish, 
  as 
  above, 
  and 
  

   thoroughly 
  treated 
  with 
  spores 
  of 
  white 
  muscardine. 
  These 
  were 
  

   from 
  a 
  pure 
  agar 
  culture 
  one 
  remove 
  from 
  growth 
  on 
  a 
  dead 
  insect 
  ob- 
  

   tained 
  in 
  the 
  field. 
  The 
  eggs 
  began 
  to 
  show 
  the 
  pinkish 
  tint 
  denot- 
  

   ing 
  development 
  August 
  10. 
  i^ugust 
  13, 
  a 
  single 
  egg 
  crushed 
  under 
  

   the 
  microscope 
  gave 
  no 
  evidence 
  of 
  fungous 
  infection, 
  but 
  August 
  14 
  

   one 
  egg 
  was 
  observed 
  having 
  a 
  profuse 
  growth 
  of 
  Sporotrichuiit 
  

   f/Iohii 
  I 
  iferum 
  coming 
  horn 
  one 
  end. 
  The 
  egg 
  was 
  completely 
  filled 
  

   with 
  this 
  mycelium, 
  as 
  shown 
  by 
  a 
  microscopic 
  examination 
  of 
  a 
  per- 
  

   manent 
  mount. 
  Two 
  bugs 
  dead 
  at 
  8:30 
  a. 
  m., 
  August 
  16, 
  and 
  hatched 
  

   since 
  10 
  o'clock 
  a. 
  m. 
  of 
  the 
  previous 
  day, 
  were 
  found 
  filled 
  with 
  

   mycelial 
  threads 
  of 
  Sporotrichum 
  (jlobuliferum. 
  August 
  18 
  two 
  

   young 
  were 
  observed 
  which 
  had 
  not 
  been 
  able 
  fully 
  to 
  release 
  them- 
  

   selves 
  from 
  the 
  egg 
  shell, 
  and 
  one 
  of 
  these 
  was 
  covered 
  with 
  a 
  ver}' 
  

   fine 
  mycelial 
  growth, 
  coming 
  principally 
  from 
  the 
  dorsal 
  surface. 
  A 
  

   dead 
  young 
  bug, 
  which 
  had 
  hatched 
  within 
  twenty-four 
  hours, 
  was 
  

   observed 
  August 
  20 
  bearing 
  a 
  profuse 
  mycelial 
  growth 
  of 
  Sporotri- 
  

   chum, 
  and 
  two 
  others 
  were 
  observed 
  in 
  the 
  same 
  condition 
  the 
  follow- 
  

   ing 
  day. 
  Seventy-eight 
  of 
  the 
  one 
  hundred 
  in 
  this 
  lot 
  finally 
  

   hatched, 
  and 
  six 
  of 
  the 
  remainder 
  exhibited 
  a 
  growth 
  of 
  muscardine. 
  

   Two 
  eggs 
  showed 
  a 
  similar 
  fungous 
  infection, 
  and 
  twenty 
  remained 
  

   unhatched 
  without 
  change. 
  

  

  As 
  a 
  check 
  on 
  the 
  foregoing 
  experiment 
  a 
  hundred 
  eggs 
  from 
  the 
  

   same 
  lot 
  were 
  treated 
  precisely 
  like 
  the 
  above, 
  except 
  that 
  they 
  were 
  not 
  

   exposed 
  to 
  fungous 
  infection. 
  These 
  began 
  to 
  hatch 
  August 
  15, 
  on 
  

   which 
  date 
  twenty-seven 
  emerged. 
  Twenty-one 
  additional 
  were 
  re- 
  

   moved 
  on 
  the 
  following 
  day, 
  and 
  by 
  August 
  18 
  all 
  had 
  hatched 
  Ac- 
  

   cept 
  eight. 
  By 
  the 
  21st 
  only 
  five 
  eggs 
  remained 
  unhatched 
  of 
  the 
  

   hundred 
  used, 
  and 
  no 
  fungus 
  of 
  any 
  sort 
  was 
  seen 
  in 
  or 
  upon 
  either 
  

   eggs 
  or 
  young. 
  

  

  August 
  22, 
  1895. 
  thirty 
  freshly 
  laid 
  eggs 
  were 
  placed 
  on 
  blotting- 
  

   paper 
  over 
  damp 
  earth 
  in 
  a 
  covered 
  glass 
  dish 
  three 
  inches 
  in 
  

   diameter, 
  and 
  treated 
  with 
  spores 
  of 
  a 
  pure 
  culture 
  of 
  Sporotrichum 
  

   glohuliferurn, 
  one 
  remove 
  from 
  the 
  insect. 
  These 
  spores 
  were 
  dis- 
  

   tributed 
  by 
  rubbing 
  them 
  over 
  and 
  about 
  the 
  eggs 
  with 
  a 
  j)latinum 
  

   needle. 
  The 
  earth 
  and 
  air 
  in 
  the 
  dish 
  were 
  kept 
  continuously 
  moist. 
  

   Developmental 
  changes 
  began 
  to 
  manifest 
  themselves 
  August 
  2(), 
  and 
  

   on 
  the 
  27th 
  one 
  egg 
  was 
  observed 
  emitting 
  a 
  conspicuous 
  felt 
  growth 
  

   of 
  Sporolrichum 
  glohuliferurn. 
  This, 
  growth 
  fruited 
  the 
  following- 
  

   day, 
  and 
  a 
  number 
  of 
  others 
  seemed 
  similarly 
  infected. 
  The 
  young 
  

  

  