﻿•>1 
  

  

  began 
  to 
  emerge 
  September 
  5, 
  ten 
  appearing 
  on 
  that 
  day 
  and 
  six 
  the 
  

   day 
  following. 
  Fonr 
  eggs 
  taken 
  at 
  random 
  from 
  time 
  to 
  time 
  and 
  

   crushed 
  under 
  the 
  microscope 
  were 
  apparently 
  not 
  infected. 
  Nine- 
  

   teen 
  young 
  emerged 
  in 
  all. 
  Four 
  eggs 
  were 
  used 
  for 
  microscopic 
  

   examination; 
  one 
  was 
  infected 
  with 
  white 
  muscardine; 
  and 
  six 
  re- 
  

   mained 
  unhatched 
  at 
  the 
  termination 
  of 
  the 
  experiment, 
  September 
  7. 
  

  

  In 
  a 
  check 
  lot 
  of 
  thirty 
  eggs, 
  kept 
  under 
  identical 
  condition 
  except 
  

   as 
  to 
  infection, 
  hatching 
  began 
  September 
  4 
  and 
  continued 
  until 
  

   September 
  7, 
  at 
  which 
  time 
  twenty-four 
  had 
  hatched 
  and 
  six 
  re- 
  

   mained 
  without 
  evidence 
  of 
  development. 
  

  

  Effect 
  of 
  Exposing 
  Chinch-hugs' 
  Eggs 
  to 
  the 
  Fungus 
  of 
  Green 
  

   Muscordine. 
  — 
  July 
  13, 
  an 
  experiment 
  was 
  made 
  to 
  test 
  the 
  possi- 
  

   bility 
  of 
  infecting 
  eggs 
  of 
  chinch-bugs 
  with 
  the 
  parasitic 
  fungus, 
  

   Entomophihora 
  aphidis. 
  For 
  this 
  purpose 
  eggs 
  were 
  removed 
  from 
  

   breeding-boxes 
  containing 
  collections 
  made 
  at 
  Tamaroa 
  July 
  10, 
  and 
  

   were 
  placed 
  on 
  damp 
  sand 
  in 
  a 
  Petri 
  dish, 
  together 
  with 
  several 
  dead 
  

   chinch-bugs 
  covered 
  with 
  a 
  fruiting 
  growth 
  of 
  Entomophtliora. 
  

   They 
  were 
  examined 
  from 
  day 
  to 
  day, 
  the 
  sand 
  being 
  kept 
  moist, 
  and 
  

   more 
  bugs 
  covered 
  with 
  a 
  fresh 
  growth 
  of 
  Entomophtliora 
  being 
  in- 
  

   troduced. 
  No 
  evidence 
  was 
  seen 
  at 
  any 
  time 
  of 
  an 
  infection 
  of 
  the 
  

   eggs. 
  On 
  the 
  contrary, 
  these 
  began 
  July 
  16 
  to 
  change 
  color 
  in 
  a 
  way 
  

   to 
  show 
  the 
  development 
  of 
  the 
  embryo 
  within. 
  On 
  the 
  20th 
  several 
  

   eggs 
  which 
  seemed 
  possibly 
  infected 
  were 
  microscopically 
  examined, 
  

   but 
  the 
  contents 
  of 
  ever}' 
  egg 
  were 
  entirely 
  healthy. 
  On 
  the 
  23d 
  

   these 
  eggs 
  began 
  to 
  hatch, 
  and 
  further 
  observations 
  were 
  not 
  recorded. 
  

  

  Experiments, 
  with 
  Entomophihoixi 
  on 
  Chinch-hugs. 
  — 
  Oppor- 
  

   tunities 
  for 
  laboratory 
  experimentation 
  with 
  the 
  Entomophtliora 
  of 
  

   the 
  chinch-bug, 
  E. 
  aphidis, 
  have 
  arisen 
  in 
  my 
  experience 
  so 
  rarely 
  

   that 
  especial 
  pains 
  was 
  taken 
  to 
  apply 
  for 
  experimental 
  purposes, 
  in 
  

   comparison 
  with 
  the 
  more 
  common 
  fungus 
  Sporotrichum, 
  some 
  ma- 
  

   terial 
  brought 
  in 
  by 
  Mr. 
  Johnson 
  from 
  a 
  field 
  near 
  Vandalia, 
  111., 
  

   September 
  14, 
  1895, 
  in 
  which 
  field 
  there 
  was 
  a 
  considerable 
  outbreak 
  

   of 
  Entomophtliora 
  among 
  chinch-bugs. 
  The 
  fungus, 
  although 
  col- 
  

   lapsed 
  when 
  brought 
  in, 
  was 
  quite 
  fresh. 
  A 
  number 
  of 
  bugs 
  adherent 
  

   to 
  leaves 
  of 
  horse-nettle 
  and 
  covered 
  with 
  a 
  growth 
  of 
  Entomoph- 
  

   thora 
  were 
  placed 
  on 
  moist 
  sand 
  in 
  a 
  large 
  jar 
  covered 
  with 
  muslin, 
  

   and 
  to 
  this 
  were 
  then 
  introduced 
  several 
  hundred 
  chinch-bugs 
  fresh 
  

   from 
  a 
  field 
  adjacent 
  to 
  my 
  office. 
  Four 
  days 
  later, 
  September 
  18, 
  a 
  

   few 
  dead 
  bugs 
  were 
  seen, 
  but 
  none 
  exhibiting 
  any 
  fungous 
  growth; 
  

   and 
  two 
  crushed 
  and 
  examined 
  microscopically 
  gave 
  no 
  evidence 
  of 
  

   Entomoi)hthora. 
  On 
  the 
  20th, 
  however, 
  six 
  days 
  after 
  the 
  experi- 
  

   ment 
  began, 
  a 
  single 
  chinch-bug 
  was 
  found 
  dead 
  and 
  attached 
  to 
  a 
  

   piece 
  of 
  the 
  corn 
  put 
  in 
  as 
  food, 
  and 
  showing 
  a 
  slight 
  external 
  growth 
  

   of 
  Entomophtliora. 
  No 
  further 
  evidence 
  of 
  infection 
  was 
  obtained 
  

   until 
  October 
  1, 
  when 
  the 
  experiment 
  was 
  discontinued 
  — 
  somewhat 
  

   hastily, 
  as 
  it 
  would 
  appear 
  from 
  observations 
  presently 
  to 
  be 
  re- 
  

   corded. 
  

  

  At 
  the 
  same 
  time 
  with 
  the 
  preceding 
  experiment, 
  two 
  ojjen 
  con- 
  

   tagion 
  boxes 
  containing 
  a 
  layer 
  of 
  earth 
  and 
  a 
  largo 
  number 
  of 
  

   chinch-bugs 
  were 
  used 
  for 
  a 
  more 
  extensive 
  experiment 
  that 
  the 
  pre- 
  

  

  