﻿100 
  

  

  placed 
  in 
  the 
  jars 
  with 
  the 
  bugs. 
  In 
  each 
  experiment 
  an 
  equal 
  

   quantity 
  of 
  chinch-bugs 
  derived 
  from 
  the 
  same 
  source 
  as 
  the 
  experi- 
  

   mental 
  lot 
  was 
  kept 
  as 
  a 
  check 
  under 
  identical 
  conditions 
  except 
  as 
  

   to 
  the 
  infection. 
  

  

  On 
  July 
  2() 
  about 
  one 
  hundred 
  bugs 
  were 
  inoculated 
  with 
  mus- 
  

   cardine 
  fungus 
  (Sporotrichum) 
  as 
  above 
  described. 
  By 
  the 
  29th 
  

   twenty-five 
  of 
  these 
  bugs 
  were 
  dead, 
  In 
  one 
  microscopically 
  ex- 
  

   amined 
  an 
  internal 
  fungus 
  growth 
  was 
  detected, 
  and 
  in 
  another, 
  not 
  

   yet 
  quite 
  dead, 
  similar 
  fungus 
  threads 
  were 
  seen 
  in 
  comparatively 
  

   small 
  (|uantity. 
  Both 
  these 
  observations 
  indicated 
  a 
  successful 
  in- 
  

   oculation. 
  The 
  remaining 
  dead 
  were 
  transferred 
  to 
  another 
  covered 
  

   dish, 
  where 
  they 
  were 
  kept 
  on 
  moist 
  sand 
  with 
  a 
  view 
  to 
  obtaining 
  a 
  

   further 
  development 
  of 
  any 
  fungus 
  by 
  which 
  they 
  might 
  have 
  l)e- 
  

   come 
  infected. 
  -July 
  80, 
  eleven 
  more 
  were 
  dead. 
  From 
  a 
  number 
  of 
  

   chinch-bugs 
  evidently 
  diseased 
  several 
  slides 
  were 
  made 
  which 
  

   showed 
  that 
  the 
  germinating 
  threads 
  of 
  the 
  fungus 
  were 
  penetrating 
  

   the 
  joints 
  of 
  the 
  legs. 
  July 
  31, 
  seven 
  more 
  were 
  dead, 
  and 
  one 
  of 
  

   these, 
  microscopically 
  examined, 
  contained 
  fungus 
  threads 
  within 
  

   the 
  body. 
  On 
  the 
  5th 
  of 
  August 
  all 
  the 
  dead 
  bugs 
  removed 
  from 
  

   this 
  experimental 
  lot 
  exhibited 
  a 
  good 
  growth 
  of 
  the 
  white 
  mus- 
  

   cardine 
  fungus, 
  with 
  the 
  exception 
  of 
  about 
  six 
  specimens. 
  The 
  

   number 
  of 
  deaths 
  in 
  the 
  check 
  lot 
  amounted 
  at 
  this 
  time 
  to 
  twelve, 
  

   on 
  none 
  of 
  which 
  was 
  there 
  at 
  this 
  time 
  any 
  apparent 
  growth 
  of 
  the 
  

   fungus 
  of 
  muscardine. 
  

  

  From 
  this 
  first 
  experiment 
  it 
  appears 
  that 
  about 
  three 
  times 
  as 
  

   many 
  bugs 
  had 
  died 
  where 
  they 
  had 
  been 
  infected 
  with 
  the 
  spores 
  of 
  

   the 
  muscardine 
  fungus 
  as 
  would 
  have 
  died 
  if 
  they 
  had 
  not 
  been 
  so 
  

   treated; 
  and 
  that 
  six 
  of 
  these 
  bugs 
  in 
  this 
  lot, 
  which 
  had 
  ceitainly 
  

   been 
  thoroughly 
  infected 
  before 
  death, 
  did 
  not 
  grow 
  the 
  muscardine 
  

   fungus 
  after 
  death, 
  even 
  under 
  the 
  most 
  favorable 
  conditions 
  it 
  was 
  

   possible 
  to 
  supjDly. 
  From 
  this 
  last 
  observation 
  it 
  would 
  seem 
  that 
  

   chinch-bugs 
  dying 
  from 
  natural 
  causes 
  other 
  than 
  parasitism 
  by 
  this 
  

   fungus 
  are 
  not 
  at 
  all 
  certain 
  to 
  afford 
  a 
  suitable 
  basis 
  for 
  the 
  growth 
  

   of 
  the 
  fungus 
  even 
  though 
  they 
  may 
  be 
  thoroughly 
  infected 
  with 
  the 
  

   spores 
  at 
  the 
  time 
  of 
  their 
  death.* 
  

  

  On 
  April 
  9, 
  1896, 
  two 
  lots 
  of 
  chinch-bugs, 
  from 
  seventy-five 
  to 
  a 
  

   hundred 
  each, 
  which 
  had 
  been 
  collected 
  from 
  their 
  winter 
  quarters 
  

   on 
  the 
  25th 
  of 
  the 
  i^receding 
  month, 
  and 
  kept 
  in 
  confinement 
  wnth 
  a 
  

   sufficient 
  supply 
  of 
  moisture 
  and 
  food, 
  were 
  separately 
  placed 
  in 
  

   glass 
  dishes, 
  one 
  lot 
  being 
  well 
  dusted 
  with 
  spores 
  from 
  a 
  corn-meal 
  

   culture 
  of 
  the 
  fungus, 
  while 
  the 
  other 
  was 
  similarly 
  treated, 
  but 
  

   without 
  inoculation, 
  and 
  ke^Dt 
  as 
  a 
  check. 
  It 
  should 
  be 
  said 
  that 
  six 
  

   bugs 
  had 
  previously 
  died 
  in 
  the 
  lot 
  from 
  which 
  these 
  were 
  taken, 
  but 
  

   that 
  no 
  trace 
  of 
  muscardine 
  had 
  appeared 
  among 
  them. 
  Neverthe- 
  

   less, 
  six 
  days 
  after 
  the 
  experiment 
  began 
  one 
  dead 
  bug 
  was 
  seen 
  in 
  

   the 
  check 
  covered 
  with 
  the 
  muscardine 
  fungus, 
  and 
  by 
  April 
  22 
  about 
  

   an 
  equal 
  number 
  were 
  dead 
  in 
  the 
  check 
  and 
  in 
  the 
  experimental 
  lot. 
  

  

  * 
  In 
  this 
  connection 
  sec 
  page 
  78. 
  

  

  