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  34 
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  branch 
  as 
  the 
  infestation 
  was 
  severe 
  and 
  in 
  such 
  a 
  position 
  that 
  the 
  

   southwest 
  rains 
  would 
  readily 
  strike 
  it. 
  From 
  one 
  to 
  a 
  dozen 
  pieces 
  

   were 
  placed 
  on 
  a 
  single 
  tree, 
  according 
  to 
  the 
  size 
  of 
  the 
  tree 
  and 
  the 
  

   abundance 
  of 
  the 
  scale, 
  three 
  or 
  four 
  being 
  the 
  commoner 
  number. 
  

   On 
  the 
  second 
  visit, 
  beginning 
  May 
  28th, 
  only 
  artificial 
  cultures 
  of 
  

   Splucrostilbe 
  were 
  used, 
  mostly 
  those 
  grown 
  on 
  corn 
  meal 
  or 
  on 
  pieces 
  

   of 
  bread. 
  About 
  a 
  half 
  inch 
  square 
  of 
  the 
  culture 
  material 
  was 
  softened 
  

   for 
  a 
  short 
  time 
  with 
  water, 
  and 
  mixed 
  witli 
  fifty 
  centicubes 
  of 
  water, 
  

   and 
  the 
  liquid 
  was 
  spread 
  with 
  a 
  sable 
  brush 
  on 
  the 
  spot 
  selected. 
  The 
  

   infected 
  spot 
  was 
  then 
  covered 
  by 
  wrapping 
  the 
  branch 
  with 
  a 
  strip 
  of 
  

   wet 
  duck 
  four 
  inches 
  wide 
  and 
  forty 
  inches 
  long, 
  the 
  wrapping 
  being 
  

   fastened 
  with 
  a 
  string. 
  These 
  strips 
  were 
  wet 
  a 
  second 
  time 
  on 
  the 
  

   following 
  morning 
  and 
  were 
  removed 
  in 
  twenty-four 
  hours 
  after 
  appli- 
  

   cation, 
  the 
  object 
  of 
  this 
  procedure 
  being 
  to 
  keep 
  the 
  culture 
  material 
  

   continuously 
  moist 
  until 
  the 
  spores 
  had 
  time 
  to 
  germinate. 
  That 
  this 
  

   was 
  done 
  was 
  shown 
  by 
  the 
  fact 
  that 
  particles 
  of 
  the 
  fungus 
  were 
  

   generally 
  whitened 
  by 
  a 
  mycelial 
  growth 
  from 
  the 
  germinating 
  spores 
  by 
  

   the 
  time 
  the 
  cloth 
  was 
  removed. 
  The 
  infected 
  spot 
  was 
  then 
  marked 
  by 
  

   a 
  white 
  string 
  for 
  convenience 
  in 
  subsetpient 
  inspection. 
  

  

  The 
  first 
  such 
  visit 
  of 
  inspection 
  was 
  made 
  at 
  Sparta 
  by 
  Mr. 
  Forbes 
  

   May 
  27th, 
  three 
  weeks 
  after 
  the 
  infected 
  twigs 
  were 
  put 
  in 
  place. 
  At 
  

   this 
  time 
  a 
  few 
  dead 
  scales 
  were 
  found 
  in 
  the 
  vicinity 
  of 
  the 
  twigs, 
  but 
  no 
  

   certain 
  evidence 
  of 
  the 
  spread 
  of 
  the 
  fungus 
  was 
  obtained. 
  On 
  the 
  next 
  

   inspection, 
  June 
  21st, 
  a 
  scale 
  dead 
  with 
  SpJuerostilbc, 
  and 
  showing 
  the 
  

   fungus 
  in 
  the 
  form 
  of 
  a 
  fruiting 
  growth, 
  was 
  found 
  on 
  a 
  tree 
  to 
  which 
  a 
  

   corn-meal 
  culture 
  had 
  been 
  applied 
  May 
  2Sth. 
  The 
  cloth 
  wrapping 
  had 
  

   been 
  accidentally 
  left 
  on 
  this 
  tree, 
  and 
  the 
  fungus 
  had 
  grown 
  under 
  its 
  

   protection. 
  July 
  6th, 
  about 
  two 
  months 
  after 
  the 
  first 
  infection 
  of 
  these 
  

   trees, 
  the 
  fungus 
  had 
  taken 
  effect 
  upon 
  adjacent 
  scales 
  in 
  practically 
  

   every 
  case 
  where 
  they 
  had 
  been 
  originally 
  abundant 
  and 
  the 
  infection 
  

   material 
  had 
  been 
  liberally 
  applied, 
  but 
  in 
  no 
  case 
  was 
  the 
  growth 
  on 
  

   the 
  tree 
  profuse 
  nor 
  even 
  generally 
  visible 
  even 
  on 
  the 
  scales 
  immediately 
  

   adjacent 
  to 
  the 
  infection 
  material. 
  Wlien 
  only 
  a 
  few 
  scattered 
  scales 
  

   were 
  present 
  no 
  start 
  had 
  been 
  made. 
  Returning 
  to 
  the 
  same 
  trees 
  

   September 
  i 
  st, 
  we 
  found 
  tlie 
  fungus 
  was 
  by 
  this 
  time 
  growing 
  vigorously 
  

   everywhere, 
  spreading 
  downward 
  in 
  some 
  cases 
  as 
  far 
  as 
  five 
  or 
  six 
  feet 
  

   and 
  on 
  lateral 
  branches 
  from 
  the 
  one 
  to 
  which 
  the 
  infection 
  had 
  been 
  

   applied, 
  as 
  far 
  as 
  a 
  foot 
  beyond 
  the 
  fork. 
  The 
  upward 
  spread, 
  however, 
  

   was 
  not 
  so 
  great, 
  the 
  spores 
  being 
  evidently 
  disseminated 
  mainly 
  by 
  

   washing 
  down; 
  and 
  tliere 
  was 
  nothing 
  to 
  indicate 
  the 
  spread 
  of 
  the 
  fungus 
  

   across 
  an 
  air 
  space. 
  On 
  one 
  tree 
  six 
  inches 
  in 
  iliameter, 
  for 
  example, 
  

   on 
  Mr. 
  Temple's 
  place, 
  the 
  fungus 
  had 
  spread 
  downward 
  about 
  two 
  feet. 
  

  

  