380 TRANSACTIOXS AND PROCEEDINGS OF [Sess. lvi. 



area be an artificial product, it will help us in our study, 

 for on account of differences in the tension, arrangements, 

 &c., of the fibrils of the hyaloplasm during conjugation, the 

 latter must assume various shapes in retracting when acted 

 upon by the same medium. 



Still studying the fig. 32, we find within the micropylar 

 nucleus (31. JV.) two homogeneous, globular bodies (^J. n.) 

 with a dark centre, and one similar body in the antipodal 

 nucleus (A.A^., p.n.). In addition to these three globular 

 bodies a number of homogeneous bodies {m) flattened out 

 against the nuclear membrane {n. m.) are seen in both nuclei. 

 The globular bodies seem to originate thus : When the 

 nuclei about to conjugate have come in contact, one or two 

 small nucleoli arise by the unequal division of the primary 

 nucleolus (fig. 45, j9. n., in the micropylar nucleus of Scilla 

 nutans). These secondary nucleoli seem to have at first the 

 power of division, but gradually they lose this power and 

 their property of becoming deeply, stained, and change into 

 globular colloid-looking masses with a central more deeply- 

 stained spot. I propose to call these bodies paranucleoli 

 because of their origin, they may always be found in the 

 micropylar nucleus and occasionally also in the antipodal 

 nucleus (figs. 32, 33 (35 ?), 36, ix n.). Fig. 33 shows two 

 nuclei flattened against one another with the approximated 

 sides appearing as an oblique septum. In fig. 34 the septum 

 runs across the conjugating nuclei at right angles to their 

 long axes, and it has begun to give way in the centre (/), 

 a point corresponding to those parts of the two nuclear 

 membranes which first come into contact with one another, 

 and which therefore have been able to react upon one another 

 for the longest time. 



The perinucleolar hyaloplasms (fig. 34) of the two nuclei 

 have just come in contact, and are seen to have apparently 

 fused in fig. 35, as the septum is still more absorbed. On 

 the left of the same figure two bodies (2? ?) are seen, which 

 may be paranucleoli, and which are separated by the septum. 

 The next step in the conjugation is an approximation of the 

 two nucleoli brought about by the antipodal nucleolus 

 travelling towards the micropylar one (fig. 36"). It almost 

 seems to have crossed the line where one would have 

 expected to find the septum in the earlier stages of conjuga- 



