394 TRANSACTIONS AND PROCEEDINGS OF [Sess. lvi. 



Strasburger gives it as his opinion that the formative 

 processes of the cell are regulated by the hyaline plasma of 

 the nucleus, the nucleo-idioplasm (chromatin of Flemming), 

 and believes that the greater the amount of this idioplasm, 

 the more readily does the division of a cell take place. 



E. Hertwig '"' also considers the chromatin of the nucleus 

 to be the most essential factor in the cell, and the carrier of 

 hereditary tendencies, and the achromatin only to play a 

 part in cell-multiplication (p. 52). 



The function of the nucleolus, according to Flemming, 

 consists in the latter acting as a special reservoir for the 

 reproduction and accumulation of chromatin. 



Strasburger considers the nucleolus to be a mass of 

 reserve-material for the nucleus, and to take no part in 

 the functions of the nucleus. 



Pfitzner believes the nucleolar chromatin during division 

 to become transformed into nuclear chromatin, and therefore 

 proposes to call the nucleolar substance " prochro matin." 



Carnoy holds that the nucleoli form a mass of reserve- 

 material for the nuclear plasma, i.e., that just as the proto- 

 plasm uses up its deposits, so does the nucleus use up the 

 nucleoli.t 



Some facts which came under my own notice are shortly 

 these : — (1) Actively budding yeast washed in distilled water 

 to remove all sugar, killed and fixed by my picro-corrosive 

 alcohol, washed and then stained with Ehrlich's acid 

 hsematoxylin, eythrosin, or eosin, shows in the youngest buds 

 a deeply-stained granule, which may be either a nucleolus or 

 a nuclear chromosome. This constant occurrence of a deeply- 

 stained granule suggests that it may be the cause of the 

 budding, i.e., of the change in the wall of the mother-cell. 



(£) A comparison of the relative position of the nucleus 

 and vacuole in the synergidffi, and the ovum shows, as has 

 already been suggested, that the synergidje receive their food- 

 supply from the apex of the ovule, while the ovum receives 

 its nourishment from the Ijasal part of the embryo-sac, 



(3) In transverse sections of chick-embryos, 48-60 hours 

 old, fixed by my m(3thod, it is constantly found that in the 

 individual cells, the nuclei are l}'ing in that part of the cell 



* R. Hertwig, Lcilirliiich d. Zoolog., 1891. 

 t Van liaiiibeke, pp. 59, 60. 



