38 TKANSACTIOXS AND PEOCEEDINGS OF THE [Sess. LXi. 



Trifoliurih pratcnsc or repcns or hyh-ichim, T. incarnatum, 

 Vicia tUIoso. and scUiva, Pisum sativum, Vicia fciba^ 

 Mcdicago sativa, M. hqnilina, Lupinv.s lutcus, L. angusti- 

 foJins, Oniithopiis sativus, Onohrycliis sativa, Lathyrus 

 sylvestris. Each bottle contains enough to supply half an 

 acre. 



In the inoculation with pure cultures there are two 

 variations — 



(a) The inocidation v:ith infected sod. Take one of the 

 above-mentioned bottles. Gently warm the bottle by 

 placing it in lukewarm water (great care must be taken 

 not to overheat — keeping in one's pocket for a few 

 minutes is enough), - and the gelatine with the culture 

 is liquified. Pour this (thoroughly cleaning the bottle 

 out) into a vessel containing sufficient water to moisten 

 half a hundred weight of soil. Mix this liquid well with 

 the earth. Then add some dry soil till a condition is 

 reached when it can be conveniently spread. Work this 

 into the field to a depth of 3 to 4 inches. Sow as soon 

 as possible. 



(i) The inocidation of the seed directly. As before, take 

 a bottle and pour the liquified contents into 1| pint of 

 water (this will do for small seeds like clover, but for 

 larger seeds use 2 or 3 quarts). Pour the mixture over 

 the seeds, and mix thoroughly, making sure that each seed 

 gets moistened. Then add some soil from the field to be 

 sown, so that the seeds don't stick together in clumps, and 

 when dry enough sow. 



In both a and h I have given tlie quantities necessary 

 to inoculate half an acre. 



HINTS AS TO EXPERIMENTS. 

 To all intending to make experiment I would recom- 

 mend that in eacli experiment, for purposes of compaiison, 

 three plots be dealt with. Let plot 1 remain uninoculated ; 

 let plot 2 be inoculated with infected soil ; let plot 3 have 

 the seed inoculated directly, Tn the case of experiment 

 on these lines, for safety let the non-inoculated plot be 

 sown before the inoculated, and, lo prevent after infection, 

 let great care be taken to have no traffic between the 

 inoculated and the non-inoculated plots. 



