126 Transactions british Mycological Soctety. 
I. MICROSCOPICAL CHARACTERS. 
The following remarks apply to all strains of Sporotrichum 
caynis used in this work, except where otherwise stated. The 
various strains are denoted either by a letter or a number. 
1. Germination of the spores. 
The spores swell considerably, each putting out one or two 
germ tubes, which give rise to a branched mycelium. 
2. Hyphae. 
The hyphae are very narrow, about Ip» in diameter, and in- 
variably hyaline. On some agar media the hyphae inside the 
medium differ from the normal type, being rather wider, and 
distinctly vacuolate. In the normal hyphae the septa are very 
obscure, but in these submerged hyphae the septa are more 
prominent. On agar the hyphae are usually straight, giving off 
branches almost at right angles to the parent hyphae. 
The few strains which grow at 30° C. form peculiar stromatic 
colonies, consisting of swollen hyphae, the cells being very short 
and almost spherical. There is no penetration of the medium 
at this temperature as there is at ordinary temperatures. 
3. Formation of conidia (Fig. 4). 
(a) Sporotrichum carnis. Aerial branches arise from the vege- 
tative mycelium, 30-50» in length. On these, other short 
branches arise, often in twos and threes, which may branch 
again. The branches are cut off by septa from the parent hypha, 
and the distal parts of the whole system of branches segment 
into short, cylindrical cells, 3-5 » long. These cells form conidia, 
the apical portion of each swelling considerably, and their walls, 
especially the transverse wall at the base, thicken. Those cells 
of the conidiophore and its branches which do not form conidia 
disorganise, leaving nothing but the walls, which are almost 
invisible. The conidia are easily detached, and on mounting a 
portion of an old colony, a mass of spores is seen, with only 
occasional portions of hyphal walls. 
(b) Sporotrichum globuliferum (from Amsterdam). As shown 
in Fig. 5, the conidiophore of Sporotrichum globuliferum differs 
greatly in appearance from that of the species isolated from 
meat. Long, aerial branches arise from the vegetative mycelium, 
many remaining sterile and contributing to the woolly appear- 
ance of the colonies, while others develop small groups of conidia 
at their ends and on short lateral branches. The conidia are both 
terminal and lateral on short sterigmata, those at the apex of 
the conidiophore being the youngest. This method of spore 
formation is distinct frem that of Sporotrichum carnis. 
