STUDIES ON Till-: I'llVSlOLOGY OK Fusarium liiiL 21 



This medium lias acid roactit)n and is cream >'elli)\v colour. The medium 

 was inoculated with the conidia or a bit of the mycelium, and was incubated 

 at zfQ. 



The growth of the nncclium is t^ood, but not rapid. The aerial myce- 

 lium grows vigorously after few days with white cottony appearance. 



The conidia are formed abundantly, and the microconidia arc more 

 numerous than macroconidia. In old cultures many chlamiilospores appear. 



The colour of the medium changes to reddish brown. 



It is one of the suitable cultural media for the fungus. 



2. Flax decoction hard agar 



Young flax plants 50 grams 



Agar 4.5 grams 



Distilled water 300 cc. 



Cook the stems and leaves of flax plants for an hour in a water bath, 

 and straining through filter paper add agar and melt. Then strain through 

 cotton and pour into test tubes. Sterilize them in the Koch's steam sterilizer 

 for an hour. The colour of the medium is pale ochre green. 



Inoculate with a bit of the mycelium or conidia, and incubate at 25°C. 

 Development of the mycelium is fair, and aerial mycelium is present. 

 Macro- and microconidia are produced equally. Ch^amydospores are formed 

 very abundantly. The colour of the medium remains unchanged. 



3. Cooked flax stems 



Take flax seedlings, about 3 inches in height, in the test tubes, and 

 heated them at iOO°C. with moisture for an hour. Inoculate them and in- 

 cubate at 25°C. 



Aerial hyphae develop moderately in a cob-web like manner. Produc- 

 tion of conidia is very good. On this medium the typical macrotype conidia 

 are produced much more than the microtype conidia. Later chlamydorspores 

 are produced, buried in the tissues. 



On the living flax stems this fungus produces sporodochia scattered, but 

 on these cooked stems the development of the mycelium is very rapid, im- 



