22 m ft"iiü m Jf. tfi f!'i m <j-> *. n. ü'i w -^ 



mediately co\criiig all over the surface of the stems and produciny the spores 

 on c\-cr)- part in diffuse manner and not in spore clumps. 



4. Potato cylinder 



It was jircjiarcd as in the method of bacterial culture Inoculate with 

 a 1)it of the mycelium or conidia, and incubate at 2yQ. 



The ni\celiuni develoiis well, and the aerial mjxelium ai)pears thick 

 and cotton}-. 



The spore formation is very p;ood. Macroconidia and microconidia are 

 produced almost in equal ciuantit>-. Chlamydospores are comparatively rare. 



The colour of the mediimi is at first white, but changes later to ochre 

 yellow 



5. Corn meal agar 



This medium was prepared as the Cook's formula. 



The mycelium creeps in the substratum, and the aerial m>'celium grows 

 poorh" in a cob-\\el> like manner. 



Conidial formation is jioor, and chlamydospores are produced abundantly. 

 The colour of the medium remains inichanged. 



6. Bean agar 



I5oir 200 cc. of ordinary soup bean in 500 cc. of distilled water for half 

 an hour until they become plump, and strain through cloth. Melt 15 grams 

 of agar in 500 cc of distilled water. Mix these two solutions, and strain 

 through cotton Place the mixture in test tubes, 10 cc. each, and sterilize 

 for two hours in Koch's steam sterilizer twice with one day's inter\'al. 



Inoculate with a bit of mycelium or conidia, and incubate at 25^C. 



The mjxelium develops fast and vigorously, the white aerial mycelium 

 covering all over the medium in 3 to 4 days after inoculation. 



Conidial formation is good, and macroconidia are produced much more 

 than microconidia. Chlamydospores are produced moderately. 



The colour of the medium remains unchanged, but a putrid smell is 

 generated. 



This is one of the good media for this fungus. 



