24 to rt- ffi » * w fn m » *. a M w '^ 



This solution Ikis weak acid reaction. Development of tlie mycelium is 

 good. Tlic aerial mycelium is imniuced vigorously on the surface of the 

 solution. 



I'drmation of the conidia is poor, the chlamj'ilospores are produced also 

 poorly. 



On the hard agar mctlium of these two solutions the fungus develops 

 in a similar manner as in the solution 



9. Miyoshi's soy agar 



The formula of this cultural medium as produced by Prof. M. Miyoshi 

 is as follc5ws : — 



Japanese soy 20 cc. 



Catie sugar 5 grams. 



]5oiled onion juice 25 cc. 



Agar 15 grams. 



Distilled water 50 cc. 



Distribute this medium in test tubes, and sterilize them in Koch's steam 

 sterilizer lor an hour and let them set. Inoculate them with a bit of myce- 

 lium or conidia, and incul)ate at 25°C. Colour of the medium is reddish 

 black and semitransparent. 



Aerial and creeping mycelium grows \igorousl}', covering all over the 

 surface of the medium in a short time. 



The formation of conidia is fair, the macro- and microconidia are pro- 

 duced e(iuall>-. Chlamydospores are very rarely formed. Colour of the 

 medium remains unchanged. 



10. S;^mthetic solution 



I have used a nutritive solution of the following formula : — • 



Ammonium nitrate (NHjNO:)) lo.o grams 



Potassium biphosphate (KH.J'Oj 5.0 „ 



Magnesium sulphate (MgSOj) 2.5 „ 



Cane sugar (CsHi-.O^) 50.0 „ 



Iron chloride (Fe^Cle) " a trace 



Distilled water (H.O) looo.o cc. 



