TOCHINAI : STUDIES ON' THE PHYSIOLOGY OF Fusariian Hid. 2$ 



This solution is colourless and transparent, and lias an acid reaction. 



Take 50 cc. of this solution in each Krlcnmeyer's flask which has 200 

 cc. capacity. Sterilize the flasks for half an hour in Koch's steam sterilizer 

 twice. When sterilization is too long, this solution change its colour to yel- 

 low. Inoculate with a bit of the mycelium or conidia, and incubate at 2 5°C. 



The cottony aerial mycelium grows vigorously, covering all over the 

 surface of the solution. Afterwards the mycelium presents a light pink 

 colour, especially when exposed to light. 



Conidial formation is very poor, and chlamydospores are produced moder- 

 ately in the old cultures. 



Colour of the medium changes to yellow gradually. 



Lack of iron salt is almost indifferent to the growth of the fungus. 



This is a good medium for this fungus, especially suited for the h}'phal 

 growth. 



11. General cultural characters 



From the results of these cultural studies with the ten kinds of cultural 

 medium, the following conclusion may be drawn. 



In saprophytic condition Fiisariinu liivi seems to be omnivorous, and on 

 the several kinds of cultural media it develops vigorously and produces 

 conidia. 



The mycelium has ordinarily aerial habit, and white cottony appearance. 

 On the corn-meal agar medium it develops, however, creeping mycelium. 

 On a diseased flax stem, we find mostly macroconidia, but on the artificial 

 cultural medium the microconidia are generally rather more numerous than 

 macroconidia. The conidia in artificial cultural media are not formed on 

 sporodochia as on the flax stem in natural condition, but they are produced 

 on loose hyphae. Therefore, we could not recognize a pink colour of the 

 spore masses on any artificial cultures, but on a certain kind of cultural 

 medium, for example, on the synthetic solution, the mycelium present a light 

 pink colour, especially when the cultures are exposed to light. This colour, 

 however, is not caused by the spore clumps, but by some pigment formed 

 in the mycelial cells. 



