TOCHINAI : STUUIKS ON THE rin'SIOI.OGV OI' Fiisatiuin Hid. 29 



In the cultures No. II, and No. 12, the <;ro\vth of tliin aerial ni>celium 

 took place on tlic .surface of the cultural medium. In No. 13 and No. 14 

 onl}- a little aerial mycelium was seen. In No. 19 there was no aerial myce- 

 lium, and the fungus appeared as a small brown mass in the bottom of the 

 solution. This brown colour was caused by the chlamydospores and 

 " Dauermycel " which have brown thick wall and granulated cell contents. 



In No. 20 to No. 22, there was hardly a trace of mycelial development. 

 The mycelium of the fungui; appeared as only a very small brown mass, 

 which ma>' probably be a little larger than when it was inoculated. After 

 three weeks, such a brown mass was taken out of the tannin solution, and 

 having been washed with sterilized distilled water several times, put in a 

 nutrient solution and incubated, then immediately it began to grow and 

 produced aerial mycelium vigorouslj-, covering all over the surface of the 

 nutritive medium, 



E.\PERIMF.Nr 2. 

 The effects of tannic acid on the germination of the conidia. 



In this experiment I have used the same cultural medium as in the 

 preceding experiment, and adopted the drop culture with the Van Tieghem's 

 cells. 



Coyer glasses were washed very carefully with acetic acid and caustic 

 soda and at last with redistilled water. 



The spores were obtained from a bean agar medium which had grown 

 for two weeks in an incubator at 25°C. 



The number of cultures was seven which contamed different percentages 

 of tannin. Ever}- number was duplicated. They were inoculated with the 

 conidia and incubated at 2 5^C. 



The results were as in the following table. 



Serial numlier % <»f tannin (jcrminatiün of conidia 



24I1. 481i. 72h. 



1 '^'h% ++ + + + + + + + + + 



2 0.6 + + + + ++■!- 



3 0.7 + ++ + + 



