30 ■ ' p5 i*j— 35 m jV. tt ?fl ffi" « ife a ft'] m '^ 



4 0.8 • + ++ + + 



5 0.9 + + + + 



6 10 — + + 



7 'o - + + 



In the culture Nii. I, after 24 hours many of the inoculated sjx>res 

 swelled anil enlarged. The contents of these spores became homojjeneous 

 and ncarl}' transparent. ]5iit only a small number of the spores germinated. 

 Some. of them shrank and their contents became granular, and died. There 

 were sjjores in which some cells shrank wliile the other cells swelled or 

 germinated. 



After '48 hours many of them germinated. Germ-tubes had grown to 

 the length of 10 to 150 microns, and some of them branched. 



After 72 hours, the nucelium liad developed to such an extent that it 

 could be seen macroscopicall}'. At this time the vigorously branched mycelia 

 produced the microt\pe conitlia at the end of the side branches. Chlamy- 

 dospores were jiroclucetl in large numbers. The shrunken spores remained 

 as before without swelling. 



In cultures No. 2 to No. 5, after 24 hours I found very few germinated 

 sjiores. The swelled spores were less in number and the shrunken spores 

 were more, the proportion between tluni \ar\'ing in accordance with the 

 increase in the percentage of tannin. 



Aftei- 48 hours, a small number of the conidia germinated, and in the 

 CLiltures No. 5 germinated spores were still more rare. 



After 72 hours, some of the well developed mycelium had branched and 

 ]5roduccd a \-ery few microconidia which are transformed directly into 

 chlamydosiiores, the Cell-wall of the conidia becoming thick and brown in 

 colour. 



In cultures Nos. 6 and 7, after 24 hours none of the spores had germi- 

 nated. Only 13 of the inoculated spores swelled more or less, there being 

 almost no spores in which all of the cells composing them swelled. 



After 4S hours a very small number of the spores germinated. 



After ~2 hours there was observed neither branched mycelium nor conidial 

 formation. Chlamydospores were Hiirly produced. Almost all of the germi- 



