3^ 



In the control culture, No. i, aerial mj-celium t^rew vigorously, covering 

 thick all o\er the surface of the niitriti\e solution. 



In No. 2 aerial mycelium grew far more poorly than No. I, covering 

 thinl}' antl deficient!}- over the surface of the nutritive solution. 



In No. 3 the fungus appeared as brown masses submerged in the solution, 

 and aerial mycelium scarceh- grew from these masses on the surfiice of the 

 nutritive solution 



In No. 4 and No. 5 the fungus was recognized only as small brown 

 masses sinking to the bottom of the solution, and there appeared on aeria! 

 myccliimi o\-er the siu-face of the solution. 



In the cultiues containing higher percentages of citric acid, above 2%, 

 I could not find the hyphal growth of the fungus macroscopically. 



The conidia were produced very sparsely in all of three cultures. 

 Chlamydospores were produced richly in almost all cases except No. I. 



In the cultures of No. 3 the greater part of the inoculated conidia shrank 

 and did not germinate. When a conidiinn germinated, the h}-pha did not 

 grow long, but its cell contents became granular, and the wall of the cells 

 thickened, coloured brown, thus changed into " Daucrmycel " or chlamj-- 

 dospore. The brown colour of the mass is due to the chlamydospores and 

 " Dauermycel." Such a chlamydospore- formation was demonstrated ev-en 

 in the cultures of No. 9 microscopically. 



If such a bro\\n mass of the fimgus-h>'phae be taken out and washed 

 well, and then brought into a new culture medium, it begins to grow and 



