34 



M i«i— ■IS I» * +,ii m m « 'k n fi'i m '^ 



The cultural media which contained above 0.5% of citric acid did not 

 soIidiK'. Jhe cultures No. i to No. 3 were used as .slants in test tubes and 

 those above No. 4 (0.5"^ citric acid ) were used in liquid form in Erlenmyer's 

 flasks. 



They were inoculated with a bit of the mycelium or conidia which were 

 produced in a culture L;rown two weeks in an incubator at 25^C on apricot 

 acjar medium. 



The results of the cultures after lO days were as in the following table. 



Twent\--four Iioin-s after inoculation, the cultures No. i to No. 3. showed 

 the visible growth of the mycelium. 



Two days after inoculation, in No. 4, No. 5 and No. 10 (apricot agar) 

 a visible growth of the mycelium had begiui. No. I to No. 3 produced 

 conidia already' a little. 



After 4 days, No. 6 and 7 began to show a \isible mj-celial growth. 

 At this time, in No. 1 to No. 4 \igorous growth had taken place, the aerial 

 mycelium covering all over the surface of the medium. No. lO was not yet 

 so good as in No. I. * 



After 5 da}s. No. 8 began to grow, but in No. 9 the mycelial growth 

 could not be seen with the naked eyes. No. i to No. 5 produced the conidia 

 richl}'. In No. 3 and No. 4 the mj'celium presented a light pink colour. 

 This coloiu" was also assumed b)' many other cultures aftcrAvard. 



