38 116 R -ffi m .V. w m m ® *. n. a^ m % 



snrfaci- of tlic incilimn. 



])ry wcii^ht of the inj'cclium ... .0.094 <^. 



9, At 36' to 37 'C. (in an incubator.) 



A liaci-. Mycelium t;revv vcij- little and did not produce the aerial 

 ni}-coliuni, sinking to the bottom of the cultural solution. 



10. At 40%'. (in an incubator.) 

 Ko growth. 



The conidia shrank and did not germinate. 

 ]''r()m these results it may be said that in this nutrient solution the minimum 

 temperature for tlie growth of the fungus is lO^ to I2'C., the optimum 

 temijcrature about 30 C. and the maximum temperature is about 36X to 37^C. 



EXPEKI.MKNT 2. 



']"he effects of a high temperature on the germination 

 of the conidia. 



] ha\e ado]ited 50^C. as tlic high temperature. The test tubes of bean 

 agar cultures of the fungus were placed in an incubator at 50'C. The conidia 

 Avere taken out from these test tul)es with an hour's interwrl, and were sowed 

 in ilrops of the nutrient solution, and incubated at 25^C. 



The Conidia which were incubated at 50*^0, for an hour about half 

 germinated, and the other half shrank ani.1 died. 



The conitlia which were incubated at 50^C. for two hours did not 

 germinate at all, the)- all shrank and were killed. 



Abü\'c three hours no conidium survived. 



Experiment 3. 

 Resistance of the fungus tp low temperature. 



I ha\e used ice and XaCl for the cooling agent. The test tubes of the 

 \igorously growing bean agar cultures of the fungus in an incubator at 25"C. 

 Avere ]ilaced in the thermos bottles which contained the cooling agent, and 

 after a certain int(.r\ al the)- were remoxed and the conidia were sown in the 

 nutrient solution and incubated at 2 5"C. The results were as following table. 



