190 ILLINOIS STATE ACADEMY OF SCIENCE 



In experiment III, several nodes of a stem were used. 

 On some stems, leaf 10, the leaves of the apical node and 

 on othe rs, l eaf 11, those of the basal node were retained. 

 The results with B. crenatum were quite different from 

 those obtained by Loeb with B. calycinum. In 5 days, 

 in leaf 10, four out of five specimens had developed basal 

 node shoots and the fifth had both basal and apical node 

 shoots. In leaf 11, there were two apical node shoots in 

 each specimen and also a basal node shoot in one. In 20 

 days, in leaf 11, there were two shoots from the basal 

 node of one specimen and some notch growth on 2 leaves. 

 After 4 weeks, leaf 10 had developed 2 shoots from the 

 basal node of each specimen. Four of the specimens had 

 developed 2 apical node shoots and also some notch 

 growth. The fifth had but 1 apical node shoot. Plate IV. 

 shows leaves 10 and 11 after 5 weeks. The growth of 

 the buds on the stem may have inhibited a vigorous 

 notch growth as stated by Loeb, but the shoots developed 

 rapidly at those nodes from which the leaves had been 

 removed, regardless of whether those nodes were apical 

 or basal. From the under side of one leaf, a shoot had 

 developed near the mid-vein. Here again we have regen- 

 eration other than that from the notches of the leaf or 

 from a definite place on the stem. 



Loeb found that in B. calycinum the development of 

 the bud on the stem was inhibited or retarded if only a 

 piece of the petiole of the opposite leaf was left on the 

 stem. This was not true for B. crenatum. In 14 days, 

 in the leaves whose opposite leaf blade had been removed, 

 leaf 14, a shoot grew from the opposite axillary bud in 

 each specimen and there was a slight notch growth on 

 most of the leaves. The petioles were still intact when 

 the shoots developed. Plate V. shows 2 specimens of leaf 

 14 after 1 month. At this time the petiole had withered 

 and fallen from one stem and a shoot was growing in the 

 adjacent leaf axil in this specimen. 



Leaf 20 on Plate V. shows the effect of cutting the 

 stem lengthwise leaving a leaf attached to each half. In 

 5 days the adjacent buds appeared on 3 out of the 6 spec- 

 imens. The other 3 showed notch growth. In 21 days 

 there was an adjacent shoot in each and a slight notch 



