K. MiYAKE : Ox THE NATURE OF THE SUOAKS FOUVD IN THE TüBEIls OF AnnOWHEAD. 29 



1. It liiid a swoctish tasto. 



2. It dill not reduce tlie Feliling's solution directly ; after iuvertion with 

 hydrocliloric acid, it nYlucod very strongl}' showing the presence of non-redncing 

 sugai-s. 



3. It did not give tlie characteristic reaction of pentose with phloroglaciu 

 and hydrochloric acid. 



4. Molish- Udi'ansky's reaction was positive. 



5. It did not give the characteristic colour reaction of Braun witli x^ici'ic 

 acid and caustic soda. 



6. It gave the characteristic fire red colour of ketose with resorcin and 

 hydrochloric acid (SeliwauofF's reaction). 



7. It did not give the charact€Tistic colour reaction of free fructose with 

 amraoniura molybdate and acetic acid. 



8. Upon oxidation with nitric acid of 1.15 sp. gr., mucic acid was produced. 



9. From the filtrate of the mucic acid crystals, saccharic acid was separated 

 and detected as acid iX)tassiura saccharate by the usual method. 



10. It did not jwoduce any crystals with phenyUiydrazme hydrochloride and 

 sodium acetate. When the mixture was warmed in a boiling water bath for 

 about 30 minutes, a few crystals of the yellowish crystalline glucosazone were 

 produced. After invertion, the gluco- and galactosazone were formed in abundant 

 quantity by heating for about 15 minutes. 



11. It rotated the plane of polarization toward the right both before and after 

 invertion ; though in the latter case its ^wwer was smaller than in the former case. 



From tlie above qualitative tests, it will be clear that the residue (I) under 

 examination contains some non-reducing sugars wliich are constituted from three 

 groux» of glucose, galactose and fructose or two of these hexoses. 



b. Isolation of sucrose. 



The residue (I) was dissolved witli 9^% alcoliol and evajwrated to a syrup. 

 It did not show any sign of forming crj'stals of its own accord, even after one 

 week's standing. An attempt was then made to purify the syrui> by meaus of 

 basic lead acetate. The syiaip was diluted with a sufficient quantity of water, to 

 which a favorable quantity of basic lead acetate solution was added and tlie 

 mixture well sliakeu. The fluid s(X)n became turbid and after standing for a little 

 while, a small amomit of flocculent precipitates was formed. After separating the 

 precipitates by filtration, a further quantity of basic lead acetate and ammonia 



