Hemmi: On a Diseasf. of some Lrgitminous Plants oausrd by CcrMophotum sctosum Kirchner \ 2X 



seem to be originated by the more or less dense formation of sliort lateral irregu- 

 lar branches composed general!)- of a few clilamydospores which adhere thnily to 

 each other forming irregular daric masses. The single chlamytlospores are glo- 

 bose, subglobose or ellipsoidal in shape, or they become often more or less angular 

 in shape b\' the mutual pressure of the adjoining cells in a knot. The chlaniydo- 

 spore-formation of the fungus was first noticed by Rostkup{8) in 1905, and Lixd(4) 

 reproduced two of the Rostkup's figures in his Danish Fungi in 191 3. 



8. Inoculation Experiments 



The inoculation experiments, from which the conclusions of the parasitism of 

 the fungus and also the identity of the fungus which attacks Lupinus polyßhyllns, 

 Cytisits ciipitatiis and one other species of the same genus have been drawn, were 

 made in the laboratory of our institution in the month of Julv of this j'ear. 



Experiment I. 



On July 4, the first inoculation test was made on the healthy leaves of the 

 seedlings of Cytisiis capltatiis., which were growing in a pot and kept on a labora- 

 tory table. Small bits of the mycelium and chlamydospores from corn-meal agar 

 cultures isolated from Lupinus polypliylliis were placed by a sterile needle in drops 

 of water on both sides of the uninjured leaves, which were previously sprayed 

 with sterilized water. To keep it moist, we placed the pot under a bell-glass 

 covered inside by the moistened filter-paper for two days. 



All of the leaves which had been inoculated showed signs of infection at 

 about the fourth or fifth day. The disease did progress i-ather rapidly, forming 

 blackish brown spots on the leaves which gradually shrunk, dried up and at last 

 dropped to the ground. But all uninoculated leaves treated in the same way as 

 controls showed no clianges for a long time. 



Experiment II. 



On July 6, the second inoculation test was made on the healthy young leaves 

 of Lupinus polyphyllus. When the plant was transplanted into a pot, all the old 

 leaves were cut off and the pot was kept in the laboratory in order to avoid the 

 natural infection. 



Small bits of the mycelium and chlamydospores from corn-meal agar cul- 



