Observations on the "Slime-fluxes" of trees. 173 



Holtz (9, p. 184) stated that he had been unable to find Torula 

 monilioides in the brown fluxes which he investigated. 



If the fungus remains entirely colourless on nutrient media, 

 as the writer's cultures seem to show, it should probably be 

 placed in the genus Oospora. 



B. Oospora {Oidium) ladis var. II. 



Strings or clumps of globular cells very similar to the cells 

 of the fungus described above may be distinguished from them 

 by the production in a hanging-drop culture of the hyphae 

 characteristic of Oospora [Oidium) ladis. Thev are not found 

 constantly in the brown flux. 



Cultural charaders. Giant colony on beer-wort gelatine after 

 ten days practically indistinguishable from that of Oospora 

 [Oidium) ladis from milk. The surface is covered by long white 

 flocculent aerial hyphae. In neutral beer-wort no fermentation 

 or surface film but a copious slimy deposit of hyphae. Xo marked 

 smeU. After two days at 26° C. acid is produced from a peptone 

 solution -f one per cent, lactose and glucose respectively*. 



Morphological charaders. Branching as in Oospora [Oidium) 

 ladis. Cells of aerial hyphae 80 x 3-6 /x. Oidia from the moist 

 area in the centre of a ten days' colony on beer-wort agar 

 average 8-3 x 2*8 ju, of the usual rectangular shape with rounded 

 ends. These may swell up before germination into spherical 

 bodies 7-4 /n in diameter. 



The young colonies are beautifully arborescent. From the 

 extreme ends of the hyphae very fine transparent filaments 

 grow down into the medium. The cells of these average 2-8 

 X 60 IX. 



The fungus is e\ddentl3' closely related to the typical Oospora 

 [Oidium) ladis of milk. 



C. Sporing Yeast. 



Accompanying the forms A and B there occurs very frequently 

 a large globular or somewhat elliptical 3'east which spores very 

 freely. It is found at all times of the year and in every case a 

 large percentage of the cells are in the sporing condition. 



Cultural characters. On beer-wort gelatine (ten days at room 

 temperature) a very moist, white, raised growth. Separate 

 colonies are perfectly round, and of a similar consistency. Acid 

 is produced from glucose after 14 days at 26° C. 



Morphological charaders. Cells from a twelve hours' beer- 

 wort culture at room temperature oblong with rounded ends 

 to globular, 5*4-9 /x x 2-y-y-2 jx. Spores eight, usually oblong 

 5-4 X 3-6 /x. Ascus membrane finely verrucose. Di\ision of cells 



* i°o Witte's peptone, 0-5% NaCl, i % lactose, etc. 



