14 TRANSACTIONS OF ROYAL SCOTTISH ARBORICULTURAL SOCIETY 



the mycelium itself is thick and fleshy, a constriction always 

 being formed where penetration of the cell walls occurs. 



No indication of digestion of fungal filaments has been 

 noted in the case of Thuja, nor have vesicular structures, either 

 attached to the mycelium or separate from it, been observed. 



3. Sciadopitys Verticillata (Japanese Umbrella Pine). 



The Japanese Umbrella pine, although it has many points 

 in common with Thuja, presents certain features not found in 

 the latter. As in Thuja, when the Endophytic fungus is present, 

 the roots are fresh, with few lateral roots developed. The cell 

 walls are free from cork wherever the fungus exists, and are 

 probably absorptive. No root-hairs are developed, and the 

 roots with the fungus within their cortical cells, have again 

 the reddish pigment, the colour being especially marked in the 

 root-tip. 



The fungus again fills most of the cells of the cortex, but is 

 not present intercellularly. The mycelium is rough and stout ; 

 cross walls are rarely present ; constrictions occur on penetration 

 of the cell walls, but not so typically so as in Thuja. 



In many cells granular masses are frequently in evidence, in 

 the centre of which is a well-defined nucleus. This resembles 

 the position found in many Monocotyledons, and may here be 

 an indication of disintegration and digestion of mycelium by 

 the Higher Plant. 



There is a marked tendency for the fungus filaments in this 

 plant to form coils (Fig. 8). 



More striking still, however, are the vesicles produced by the 

 mycelium (Figs. 8 and 9). These vesicles are terminal and no 

 cross wall cuts them off from the hyphae. They have granular 

 contents and thick walls. In many cases, however, round 

 circular bodies occur within the cortical cells, quite separate 

 from the fungal strands. These structures have a thick cell 

 wall, granular contents, and oval bodies within, varying in 

 number from 3-5. The whole structure resembles that found 

 in the spruce. What they are is not known. Examination of 

 the roots of Japanese Umbrella pine plants has revealed the 

 fact, that this plant under certain conditions produces, not 

 the Endotrophic form, but the Ectotrophic, in which case all 

 structures resemble those already described for spruce. 



