ON THE SO-CALLED BLACK-HEAD OF TURKEY.i^ 



By 



Y. Wataxabe. 



Agricnitimil College, Tohokii Imperial University. 



Iiitnxluctorv. 



Since 1905, tlie turkevs in our college farm have greatly been destroye<l bv 

 sc>m' unknown disease. When attacked bv the disease in question, they fell within 

 a ft-w day-. Tlie disease seems to be infectious, and esj^ecially perilous. (^ur 

 farm keeper threw some of the dead animals into Av^ak alcohol, probably weaker 

 than IQoo. I have undertaken a work to determine the cause of the mischief 

 and, if jxvssible, to find out the methods in taking care of tlie suffering in- 

 dividuals. The present paper is embxlied of the results so far as obtained in this 

 work. 



The material fixed in the above-.-i:ated fluid was put in successively .■=tronger 

 alcohols and was imbedded in the ordinary way in paraffin-mixture. It was 

 next divided into sections about 1/lCMl mm. thick, making use of the Schanze's 

 microtome. 



As regards the staining fluids, I employed several kinds, such as Delafield's 

 hfematoxyline, Hansen's hjematoxyline, eosin, congo-red, etc., but double staining 

 by Hansen's hrematoxyline ccimbined with congo-red proved to be the best. For 

 the staining of bacteria, which are found among the ccecal contents, fuchsin, 

 gentiana-violet, methyl-blue and Gram's fluid were preferable. 



To mv gnat regret, the present material is very imperfect, indeed, owing 

 doubtless t«i the imperfect method of fixation. As very well known, tissues tlirown 



1 This paper wa> originally presented a.s a graduating dissertation. 

 [Traas. i^c-ip. Nat. lU<x. S<x-.. V-.l. III. I'.HO.] 



