Some Tomato Diseases. F. T. Brooks and G. O. Searle. 175 
originally collected at Detroit, Michigan from a cucumber grown 
in Florida. To date no fructifications have formed on any of 
the media used so that it has not been possible to make a 
single-spore culture. 
The herbarium specimens examined for the purpose of this 
investigation were as follows: 
1. Mycosphaerella citrullina on tomato stem collected at 
Cheshunt, June Ist, 1911, kindly supplied by Mr A. D. Cotton, 
Ministry of Agriculture, Herbarium No. A 14405. 
2. Mycosphaerella citrullina on tomato stem collected at 
Finchley, July 1915. From the same source as No. 1, Herbarium 
No. A 25208. 
3. Mycosphaerella citrullina (C. O. Sm.) Gross. on water- 
melon stem, collected at Lakeland, Fla., U.S.A., in April 1919, 
kindly sent by Dr C. L. Shear. 
4. Mycosphaerella citrullina (C. O. Sm.) Gross. on water- 
melon from Florida, collected in New York market, May 1919; 
also sent by Dr C. L. Shear. 
5. Mycosphaerella citrullina Gross. on tomato stem collected 
by A. A. Wills at Cheshunt, September 16th, rgro; in the Kew 
herbarium (6). 
6. Mycosphaerella citrullina Gross. on tomato stem collected 
by F. Hearnum at Waltham Cross on June 14th, 1909; in the 
Kew Herbarium. 
7. Mycosphaerella citrullina Gross. on the lower part of melon 
stem, collected by I. W. Read at Norwich, September 1912; 
in the Kew Herbarium. 
8. Phoma Lycopersici Cke. on tomato stem. Collected by 
J. E. Vize (circa 1884?) ; in the Kew Herbarium. 
g. Diplodina citrullina (Sm.) Gross. Collected in Branden- 
burg ‘‘auf trockenen Ranken von Cucumis sativus” by P. Vogel, 
December 18th, 1908; from Sydow’s “‘Mycotheca germanica” ; 
in the Herbarium of the British Museum. 
10. Mycosphaerella citrullina (C. O. Sm.) Gross. on cucumber 
fruit collected in Florida, May 1917. Received from Dr C. L. 
Shear (s). 
An effort was also made to obtain Plowright’s type specimens 
of Phoma destructiva and Sphaeronema Lycopersici(9) but these 
were not forthcoming*. 
A detailed investigation of the cultural and other character- 
istics of the various forms isolated was commenced in August 
1919. In the preliminary cultural trials “‘pure cultures’’ were 
used but not “‘single-spore”’ cultures; single-spore cultures were 
* We are indebted to Mr W. B. Grove for kindly searching for these speci- 
mens in Plowright’s herbarium, which is now in the Botanical Department of 
Birmingham University. 
