176 Transactions British Mycological Society. 
isolated later and were used throughout the remainder of the 
investigation. The cultures and inoculations were made and 
maintained at ordinary laboratory temperatures. — 
PRELIMINARY INOCULATIONS AND CULTURE 
EXPERIMENTS. 
A preliminary series of inoculations and culture experiments 
were carried out, using the various forms as isolated on tomato 
agar. Twelve forms were used, namely: A, B, C, D, E, F, G, 
H, J, K, L, M, which were the only ones available at the time. 
The following hosts and artificial media were employed with 
a view to obtaining a general idea of the characteristics of the 
various forms and to discover, if possible, some medium which 
would give a maximum spore formation, before proceeding to 
the isolation of single-spore cultures and a final comparison of 
the various forms. 
Series A consisted of inoculations into nearly ripe tomato 
fruits kept in a large glass dish in the laboratory. Two cuts 
were made with a sterile scalpel in each fruit and a fragment 
of mycelium introduced from each culture. Control tomato 
fruits were cut but not inoculated. 
In each case a rot of the fruit commenced by the fourth day, 
except in the controls which remained healthy. All the forms 
were then successfully re-isolated on to tomato agar from the 
fruits. Descriptions of the appearance of these fungi on tomato 
fruits are given later. 
Series E consisted of inoculations of pieces of petiole of 
vegetable marrow, sterilised and kept in tubes at laboratory 
temperature. On this substratum all the various forms grew 
luxuriantly and spores were freely produced. 
Series G consisted of inoculations into stems of living tomato 
plants in a greenhouse. Mycelium was inserted into a slit in 
the stem and the wound was then bound up with tin-foil. In 
the case of Form L a large canker was formed about 24 inches 
long, which spread also to the base of adjacent branches. Large 
numbers of pycnidia were visible, and re-isolation on to tomato 
agar was carried out giving Form L again. In all the other 
inoculations there was a slight discoloration of the stem, but . 
no cankers were formed nor was there any trace of pycnidia. 
Series K consisted of inoculations on to sterilised potato cores, 
Series L on to sterilised potato cores plus 1 % glycerine, and 
Series M on to potato agar; these three media were used to 
try to discover one which would give a uniform production of 
fruiting bodies of the various forms. However, none of them 
was particularly successful, though all the various forms de- 
veloped on these media to a greater or less extent as far as the 
