260 Transactions British Mycological Soctety. 
The additional facts just described, which again show that 
monosporous mycelia produce clamp-connections and fruit 
readily and perfectly, afford strong confirmatory evidence of 
the correctness of the conclusion to which I cdme in my first 
paper, namely, that Coprinus sterquilinus is homothallic. 
V. COPRINUS STERCORARIUS. 
In a new series of monosporous cultures of Coprinus ster- 
corarius, clamp-connections were formed: (I) on each of three 
mycelia which originated from single spores of a wild fruit-body, 
and (2) on each of six mycelia which originated from single 
spores produced by a fruit-body of monosporous origin. All 
these nine mycelia produced clamp-connections two days after 
isolation and transference to a new plate. They therefore be- 
haved exactly like the monosporous mycelia described in my 
first paper. 
Three of the mycelia of the second series (2) were allowed to 
continue their development on dung-agar plates, and there they 
produced small perfect fruit-bodies which shed spores. It thus 
was proved that monosporous mycelia of Coprinus stercorarius, 
even of the second monosporous generation, are able to fruit in 
a perfectly normal manner. 
The additional facts just described, which again show that 
monosporous mycelia produce clamp-connections and fruit 
readily and perfectly, afford strong confirmatory evidence of 
the correctness of the conclusion to which I came in my first 
paper, namely that Coprinus stercorarius is homothallic. 
Brefeld* shows clamp-connections as occurring on a mono- 
sporous mycelium of C. stercorarius. His observations and my 
own are therefore in accord. On the other hand, Kniepf, without 
giving details of his evidence, states that this fungus is hetero- 
thallic. It is, therefore, possible that there may be in existence 
both homothallic and heterothallic strains of C. stercorarius. 
Further experiment with diverse strains obtained from different 
localities can alone teach us the truth about this matter. 
VI. CopRINUS LAGOPUS. 
In the series of experiments upon Coprinus lagopus recorded 
in my first paper I found that clamp-connections developed: 
(I) on each of several mycelia of monosporous origin derived 
from the spores of a wild fruit-body, (2) on each of three mycelia 
of monosporous origin derived from spores produced by a fruit- 
body of monosporous origin, and (3) on a compound mycelium 
* O. Brefeld, Untersuchungen, Leipzig, Heft 111, 1877, p. 206 under Fig. 3 b; 
also Taf. 1, Fig. 3 b. 
t Hans Kniep, Joc. cit. p. 13. 
