226 ILLINOIS ACADEMY OF SCIENCE 
irregular in size and shape depending upon the number held 
together by the gelatinous substance. The older the colony 
the larger the clusters were; a tube four months old showed a 
cluster 1 cm. long and 2 cm. wide. 
No difference could be noticed in the size or shape of the 
spores or the mycelium on the different media used; but the 
number of spores produced, the size of the spore clusters, and 
the abundance of the mycelium varied. When double the 
amount of asparagin was in the synthetic medium used the 
spores and mycelium were abundant. In media with double 
the usual amount of glucose the growth of mycelium was large 
but the number of spores produced was few. On synthetic 
media without asparagin the culture was weak and spread over 
a large surface. The same was true when no glucose was 
present. On plain agar, the development was weak and growth 
ceased entirely after the colony reached the size of 2 cm. 
INOCULATIONS 
When inoculated on healthy apples the fungus produced a 
characteristic spot. The development was slow, about two 
weeks being required to develop a distinct spot. In many 
cases no infection took place. The spot, when produced, was 
light brown with a distinct margin, which was slightly darker 
than the rest of the diseased portion. After about a month the 
spot was sunken and had not developed over 1 cm. in diameter, 
The surface was slightly hardened. 
In cross-section the spot showed, in many cases, a diseased 
portion of much larger area than could be seen superficially. 
The fungus had, in one case for example, penetrated almost 
into the core of the apple in three weeks. It made a rather 
soft spongy rot—not as soft, however, as that of Penicillium. 
While the surface spot in this case was only .5 cm. in diameter, 
the tissue below was rotted 2 cm. deep and 1 cm. iri either 
direction from the spot. In other cases the rot was not so 
rapid—penetrating only about .5 to 1 cm. with a surface spot 
of .5 cm. after two weeks. No observations have been made 
on fruit which had been inoculated more than a month. 
