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rent forms observed. It seems that only the spireme stage 
of the prophase has been stained. In the other species of 
Botrychium, we found nothing to suggest an explanation of 
this curious fact. 
In one case a young secondary root showed a quite con- 
spicuous apical cell, very much of the kind described by 
Campbell and Jeffrey for B. virginianum. The advanced 
stage of this root, however, made it impossible to determinew 
hether this root originated from the endodermis, as van 
Tieghem states is the case in ferns, or from the pericycle, 
as is the case in Angiopteris and in Todea barbara, recently 
investigated by Cribbs. 
One peculiarity of which we found no statement in the 
literature was the fact that, at very young stage, the leaf develops 
stomata. (Fig. 46). This leaf was most probably rudimentary. 
The stoma observed was conspicuous by the thickness of its 
cuticle and its rather primitive shape. In the same figure the 
crushing of the subepidermal layer of the older leaf can be 
observed. Only the tannin cells of this layer have sufficiently 
sclerenchymized walls to withstand the pressure. 
The study of B. neglectum brought out the following facts: 
1. There is a very close similarity between B. simplex and 
B. neglectum. 
2. The leaf trace, however, shows an increasing tendency 
towards doubling. The same fact holds for the root trace. 
3. The occurrence of the tannin cells is even more wide- 
spread than in B. simplec. 
4. The chromatin of many embryonal cells assumes a black 
color. 
5. The apical cell resembles that of Danaea. 
6. There were only two cells in the vascular system, whose 
origin was not certainly phytonic. 
