145 



possible external infection ^). With a knife, sterilized in a 

 flame, the bark was eut away and small pièces of the 

 mycélium were placed in Pétri dishes on an agarsubstrate 

 of the folio wing consistence: 



water 



agar VI,^2'U 



glucose V20 7o 



peptone V20 7o 



KH.PO, 'i,ru 



acid. lacticum 1 7o ^- P^o 100 ce agar. 

 On this substrate the growth of the fungus was very 

 slow; from the white or yellowish bits of the mycélium, 

 root-like rhizomorphs grew into the agar; thèse were 

 white at first, then brown, finally black, and from older 

 cultures in high Pétri dishes they often grew out of the 

 agar. In most cases only very scanty mycelium-growths 

 were visible on the agar itself, except for a hard brown 

 crust of mycélium. On more concentrated agars, damped 

 rice, bread etc. the growth was somewhat accelerated, 

 yet it maintained a slow process. In the substrate an 

 abundant growth of brown or black rhizomorphs appeared, 

 but on the substrate only very little mycélium was seen, 

 it in most cases being brown in colour. 



I tried several kinds of substrates, both liquid and solid 

 kinds, but I will hère not dwell upon thèse, because during 

 my stay in Java, I was unsuccesful in obtaining fruit 

 bodies. On my return to Holland in 1916 I brought some 

 pure cultures over with me, and thanks to the kind 

 assistance of Professor Dr. F. A. F. C. Went, I was 

 permitted to cultivate the fungus in the Botanical Laboratory 

 at Utrecht. On the advice of Miss Cath. Cool of the 

 Leyden Herbarium, I cultivated the fungus in large 

 Erlenmeyer flasks filled with: 



') cf. A. Rant. Der graue Wurzelpilz von Cinchona 1. c. p. 5. 



