SPIROCHMTA BALBIANIL AND SPIROCHHTA ANODONTA. 35 
base of each terminal process or filament in favourably stained 
specimens a small red dot or fine granule is seen (Pl. 3, 
fig. 38, b.g.). These are the “ basal granules.” Strictly I think 
the ends of 8. anodonte are quite alike and that each one 
is pointed,! especially after looking at living specimens. But 
in some stained preparations one is apt to get specimens with 
one end a little more rounded than the other (PI. 2, fig. 34), 
such differences possibly result during the making of the stained 
preparation, and are probably neither natural nor normal. 
The significance of the terminal processes of S. anodontx 
is interesting. They seem to be stiff. The membrane is 
probably directly continuous with them, at any rate the pro- 
cesses are directly continuous with the periplast, indeed, are 
a part of it. Are they chromatin-containing flagella, the 
fine prolongations of the chromatin body of the membrane ? 
This is a difficult question when dealing with such minute and 
fine structures. I prefer to leave the question still open, only 
expressing an opinion that they do not appear to be motile 
flagella, and that S. anodonte is strictly a Spirochete, 
possessing pointed ends. 
The ends or membrane of 8. balbianii are not thus pro- 
longed. In this species the membrane does not extend quite 
to the ends of the body by a distance of 2m to 3yu. S. 
plicatilis also possesses rounded ends. 
No flagella could be detected in the case of S. balbian1i, 
even after the use of appropriate reagents, such as tannin. 
It may be of interest to mention that specimens of Try- 
panosoma gambiense, stained with gentian-violet, did 
not exhibit any fibrils in the membrane, nor markedly on the 
surface of the body. Gentian-violet, indeed, was not a suc- 
cessful stain for showing the structure of T. gambiense—it 
was not precise, but vague and diffuse. The appearances of 
the membrane, after staining with gentian, were quite differ- 
ent in the cases of 8. balbianii and T. gambiense. It 
might, even, be not too wild to consider whether or not this 
1 In 8. duttoni one end is said to be pointed while the other is rounded 
(12). 
