SPIROCHETA BALBIANII AND SPIROCHETA ANODONTH. 61. 
cipitate—while a violet coloration resulted from a mixture 
of copper sulphate and potash (Piotrowski’s test). 
The style is soluble in water, giving a definite alkaline 
solution, not neutral as stated by Mitra. The style is also 
soluble in dilute solutions (up to 5 per cent.) of sodium 
chloride and Epsom salts (magnesium sulphate). Its concen- 
trated solution in water coagulates on heating, and from such 
concentrated solutions it may be precipitated by nitric acid, 
and by alcohol. It is, however, insoluble in concentrated 
saline solutions, such as those of sodium chloride and magne- 
sium sulphate, and is also precipitated by tannin. It is then 
a globulin. A portion of liver of the Anodon ground up in 
saline solution also gave these reactions, as did a piece of the 
“fléche tricuspide ”’ of Poli, though, in these cases, the test- 
results were less marked. 
Mitra states that the style is entirely soluble in water, and 
this has been queried by Latter (27). I find that a style of 
average size (about two inches long) is dissolved in from 
three to four hours in water from the medium in which the 
Anodon is kept. Occasionally complete solution may require 
from five to six hours. Mitra gives no statement of the time 
required for solution. During this period of solution the 
Spirochetes swim about actively. 
Several styles from different mussels were added together 
to a little starch solution, and after reacting for some time— 
preferably two to three hours—gave a sugar capable of 
reducing a cupric solution to the cuprous state. An amylo- 
lytic ferment is, then, present in the proteid substance of the 
style. 
The crystalline style, is, according to Mitra, secreted by 
the liver, but it may also be partially secreted by the yellow, 
granular, epithelial cells lining the stomach and intestine. I 
think these cells of the wall of the stomach undoubtedly 
secrete the “ fléche tricuspide” or cuticular lining of that 
organ. 
The style in the oyster, like that in Anodon, lies in the 
cecal division of the anterior part of the intestine, separated 
