SPIROCHATA BALBIANII AND SPIROCHATA ANODONTA. 71 
hand end, while in the remainder of the body the chromatin is disposed in 
rodlets. The basal granules are not well stained. Gentian violet. x 1500. 
Fic. 15.—Parasite in process of longitudinal fission, the membrane having 
already divided into two daughter membranes. Spiral nuclear filament well 
seen in the middle region of the body. Basal granules faintly stained, showing 
division. Gentian-violet. x 1500. 
Fie. 16.—Longer, rather straight form showing division of membrane. 
Spiral nuclear filament at one end, where the basal granule has just divided. 
Basal granule has not yet divided at opposite end (which is unusual). Gentian- 
violet. x 1500. 
Fie. 17.—Longitudinally divided parasites in process of separation. Two 
basal granules can be seen at the undivided end, while there is a single basal 
granule at the free end of each daughter form. Nuclear detail not clearly 
distinguishable. Iron Hematox. x 1000. 
PLATE 38 (Lert Hatr). 
Fic. 1§.—Daughter forms, still attached at one end, and vibrating in 
unison, arranged like the legs of a compass. A vacuole is seen in the cyto- 
plasm of the common area of attachment of the two parasites before the 
longitudinal division is completed by the final separation of the daughter 
individuals. Leishman’s stain. x 1000. 
Fic. 19.—Long form, with the membrane discontinuous in the centre, 
at any rate along one side, where there occurred a vacuole-like area with 
clear and sharp contour. The periplast was continuous over the gap, at any 
rate definitely on one side. ‘This seems to be a case of transverse division. 
The original basal granule at either end of the transversely dividing form has 
in each case divided into two. The whole nucleus seems in process of 
division longitudinally, or preparing for such division. Hach ‘ transversely 
produced ”’ daughter form seems about to divide immediately by longitudinal 
fission. Further, reddish or pinkish dots are seen in the periplast on the 
edge of the body in at least two places, and these are apparently myonemes 
—slightly contracted—in the surface of the periplast of the body proper. 
Billet’s stain. x 2000. 
Fre. 20.—Specimen, stained with Billet’s modification of the Romanowsky 
stain, showing the edge of the membrane folded over in the middle portion. 
Basal granule at each end. X 1800 approx. . 
Fie. 21.—Somewhat coiled specimen, which is small, but nevertheless 
shows membrane and basal granules. Billet’s stain. x 1000. 
Fig. 22.—Typical parasite, probably killed (with osmic vapour) while in 
rapid forward motion, judging from wave-like outline of body and membrane. 
Basal granule (4.g.) clearly distinguishable at either end, and perhaps a 
