166 E. A. MINCHIN. 
by the fact that the fat-globules float upwards, while the 
salivary corpuscles lie at the lowest focus. The Malpighian 
tubules contain coarse, yellowish-brown ‘granules similar to 
those found in the proctodeum. Neither in the salivary 
glands nor in the Malpighian tubules did I ever find trypano- 
somes or any bodies other than those already mentioned. As 
I have already stated, the statement of Gray and Tulloch (17), 
to the effect that they found trypanosomes in the salivary 
gland must be corrected; I was present when they made 
the observation, and saw the preparation; by salivary gland 
was meant proventriculus. . 
After the Malpighian tubules I next examined the genitalia, 
either testes and seminal vesicles, or ovaries, receptacula, and 
oviducts (including uterus and embryo, if present). Here 
also I found in no case anything but the proper contents of 
these organs, which I need not describe in detail. 
The last process is to dissect out the gut for its whole 
length and to divide it into its four regions, namely (1) the 
proventriculus with the thoracic intestine and sucking 
stomach, (2) the stomach with the red blood, (3) the intes- 
tine with the black blood, and (4) the proctodeum and 
rectum. In none of the flies dissected by me did I find 
trypanosomes in the proventriculus, but Gray and Tulloch, 
with their more abundant material of T. grayi and 'l. tul- 
lochii, frequently found these species in this part. No 
trypanosomes were ever found in the sucking stomach,' 
which in the normal condition is filled with air, but which 
immediately after feeding is found to contain traces of blood, 
and is sometimes quite full of blood, though this is a rare 
condition, and perhaps the result of some functional derange- | 
ment of the organ. The fly, when feeding, appears to fill 
the sucking stomach with blood, and then to expel it thence 
into the digestive tract. It can be observed that when suck- 
ing blood the fly at intervals raises its head up a little, thus 
’ Gray and Greig (18) report trypanosomes in the “ ventral food reservoir ” 
(sucking stomach ?) twelve hours after feeding. Stuhlmann (41) prefers the 
name “ crop” for the sucking stomach. : 
