192 BE. A. MINCHIN. 
split in two (figs. 206, 207). This crossing of the flagella 
marks a second characteristic stage during which the division 
of the kinetonucleus takes place. The splitting of the 
flagellum proceeds a certain distance and then breaks off, so 
that a longer and a shorter flagellum result from it (figs. 208— 
210). About the time the splitting of the flagellum is com- 
plete the nucleus divides. I have found stages of the division 
in which a thread or band ran between the two daughter- 
nuclei, connecting two granules, doubtless of centrosomic 
nature, imbedded each in the mass of one of the two daughter- 
nuclei, and difficult to distinguish from the chromatin 
granules surrounding them (figs. 209, 210). As may be seen 
from the figures, there is the utmost diversity in the relative 
positions of the two nuclei and the two kinetonuclei. I must 
therefore retract my former statement (P. R.S., B 78, p. 243) 
as to the constant position of n and N after division. When 
the division of the nucleus and flagellum is complete, the 
body begins to split (figs. 209-212), starting from the 
anterior extremity between the two flagella. The body 
always divides into two unequal portions, giving rise to 
individuals which are markedly unequal in size and may be dis- 
tinguished conveniently as parent and daughter respectively. 
The parent takes the longer flagellum, that is to say, the 
principle portion of the original flagellum; the daughter 
takes the shorter flagellum. The two individuals are attached — 
last in the neighbourhood of the kinetonucleus (fig. 212; 
compare fig. 217), until they finally break loose. 
By some authors it has been stated that, in the division of 
trypanosomes, the daughter flagellum is not split off from the 
parent, but grows out independently of it. I have paid much 
attention to this point in T’. grayi, and can find nothing to 
indicate that the new flagellum is formed otherwise than I 
have stated above, namely, by a process of splitting which 
starts from the division of the blepharoplast. At the same 
time I can quite well imagine that in other species the 
process may take a different course. It is seen that even in 
’. grayi the splitting does not extend to the whole length 
