226 BE. A. MINOHIN. 
is the record of the infected monkeys used by me, copied 
from the records of the Sleeping Sickness Commission, and 
kindly supplied to me by Gray, who entered them in the book. 
“Monkey 404. 
“June 7th, 1905.—Inject 5 e.c. of cerebro-spinal fluid from 
case of sleeping sickness, ‘Sengoma.’ Active trypanosomes 
are present in this fluid. 
“July 16th—Trypanosomes have not appeared in this 
animal’s blood. Re-inject animal with a few drops of blood 
from Monkey 420. ‘Trypanosomes are numerous in the blood 
of this latter animal (see Pl. VIII, figs. 22-25). 
“ Aug. 2nd.—Trypanosomes have appeared in this animal’s 
blood to-day for the first time. 
“Sept. 9th.—Trypanosomes have been regularly present 
in this animal’s blood up to now. To-day the animal was sent 
to Nairobi at the request of the P. M. O. for the use of Dr. 
Ross, who is about to conduct some experiments on trypano- 
some transmission with the local tsetse-flies.”’ 
“Monkey 420. 
“ May 23rd, 1905.—Inject this monkey subcutaneously with 
2 c.c. of cerebro-spinal fluid obtained from case of sleeping 
sickness, ‘ Vikitikeza.’ Trypanosomes are numerous in this 
fluid. 
“ June 7th.—Trypanosomes have appeared in this animal’s 
blood to-day for the first time. The parasites are scanty in 
numbers, and are long and thin for the most part. 
“ July i3th— Trypanosomes are numerous in its blood. 
“Sept. 18th—The animal died to-day. Trypanosomes 
have been constantly present in its blood from the begin- 
ning. Post-mortem examination revealed nothing of 
interest.” 
