932 E. A. MINOHIN. « 
Oct. 6th (seventy-two hours).—One fly was. dissected 5- 
there was no blood in the digestive tract, only some 
brownish fluid in the intestine, in which two trypanosomes 
were seen. One smear was made, but no trypanosomes 
could be found in it. J) 
Batch of Oct. 9th, 1905.—This batch consisted of 
two flies, both of which had been bred from pupe in August, 
and had been kept since then in a fly-cage and fed regularly 
every two or three days on a fowl. The. same fowl had been 
used for feeding the flies in the breeding-cage—that 1s to 
say, flies caught wild near Entebbe and kept in a large cage 
in order to obtain pupe from them. The fowl became 
emaciated and of sickly appearance and died ; its place was 
then taken by a second fowl. Unfortunately the. fowl that 
died was not examined. 
On Oct. 9th the two flies were fed on Monkey 409, 
which was showing a fair number of trypanosomes ('T. 
gambiense) in its blood. 
Oct. 10th (twenty-one hours after infection). — One of 
the flies were dissected. Both stomach and intestine were 
full of red and black blood respectively, and both were 
swarming with Trypanosoma grayi. In the proctodeeum 
a few non-motile slender trypanosomes, apparently dead, were 
seen. No trypanosomes were found in the pericardial fluid, 
salivary glands, proventriculus, thoracic intestine, or sucking 
stomach. Hxamination of smears gave the following results: 
In all I. grayi was abundant, but prolonged searching was 
necessary to find T. gambiense, a specimen of which, 
from this fly, was figured by me (P. R. S., B78, Pl. XU, 
fig. 14). 
The smears of the red blood showed great variety of forms. 
The following types could be distinguished: (1) large forms, 
with hinder end narrow (figs. 218, 219) ; (2) large forms, with 
hinder end swollen, apparently about to divide (fig. 221) ; 
(3) quite small forms (fig. 220); (4) medium-sized, apparently 
representing stages in the growth of (3) into (1) and (2) ; (5) 
round forms, connected by transitions with (3) (figs. 222, 
