234 KH. A. MINCHIN. 
anemic, and its digestive tract contained no blood, but 
swarms of large bacteria. No trypanosomes were found. 
Batch of Oct. 15th.—A number of flies, freshly caught 
near Entebbe, were fed upon Monkey 478. 
Oct. 16th.—Four flies (one female, three males) were dis- 
sected, at about twenty-one, twenty-four, twenty-seven, and 
twenty-eight hours after infection respectively. In all of 
them trypanosomes were found both in the red and black 
blood, not very abundantly, but slightly more so in the black 
blood. They were for the most part of stout form, but some 
slender ones were seen also. In the black blood from the 
second fly two trypanosomes were seen adhering together by 
the hinder end; after a time a third added itself to them. 
Smears were made from all the flies, both of red and black 
blood ; they showed trypanosomes rather scanty im number, 
but sharply differentiated into slender and stout types (figs. 
45-47); very few dividing forms were seen. In one smear, 
33 stout and 4 slender were counted ; in another, 88 stout, 4 
slender, and 3 dividing. 
Oct. 17th.—Five flies were dissected. The first fly (male, 
forty-five hours after infection) was plump and fairly full of 
blood, both red and black. ‘Trypanosomes were seen fairly 
abundant in the red, still more so in the black blood; in the 
latter many of them were united by the posterior end into 
groups of as many as five. As these trypanosomes appeared 
active and healthy, as well as numerous, the experiment was 
made of injecting some of both red and black blood into a 
monkey (No. 509), but the injection was without effect, and 
the monkey never became infected (see p. 227). 
Smears of the red blood showed trypanosomes sharply 
differentiated into stout and slender forms (figs. 76, 77), 
similar to those found the day previous. Smears of the black 
blood, however, showed a slightly different type, moderately 
stout, rather long, and characterised, for the most part, by 
considerable length of the free flagellum (figs. 73-75). 
The second fly had the gut perfectly oe of blood, and 
contained no trypanosomes. 
