TRYPANOSOMES IN TSETSE-FLIES AND OTHER DIPTERA. 239 
was found swarming with T. grayi. Five smears were made, 
but unfortunately no note was kept as to the parts of the gut 
from which the smears came—an oversight much to be 
regretted, as the preparations show trypanosomes of a remark- 
able type; slender ones varying from small (figs. 235, 236) 
and medium-sized (fig. 241) to a very great length (figs. 
242-244). In some of the very long ones » could not be 
made out clearly, perhaps on account of defective preserva- 
tion; in others » distinct. Large forms also occur (fig. 238), 
and a form with N posterior (fig. 234) is also common. In 
nearly every case n is Well in front of N; only occasionally 
n at the side, and in two cases noted to be behind NV. A few 
dividing forms were seen. In the preparations fixed with 
absolute alcohol the trypanosomes frequently appeared very 
granular, and full of chromidia. 
Batch of Nov. 3rd, 1905.—A batch of Glossina pal- 
palis, freshly caught, was fed on Monkey 478 (see p. 227), 
which was showing a fair number of trypanosomes in its 
blood. Smears were made at the same time of the blood 
of the monkey, ‘The batch of flies was re-fed on healthy 
animals on the 5th, 7th, and 8th, and after that daily, until 
the flies had all been used up for dissection and examina- 
hiom. 
The smears of the monkey’s blood showed trypanosomes 
fairly abundant and of various types (see figs. 14-17). Of 
212 trypanosomes counted, it is noteworthy that only one 
was dividing ; the remainder comprised the following types 
of form: stumpy forms (figs. 16, 17), 82 (= 38:1 per cent.) ; 
long forms (fig. 14), 63 (= 31:2 per cent.); imtermediate 
forms, slender, 28 (= 12°6 per cent.); intermediate, broad 
(fig. 15), 38 (= 17:5 per cent.). ‘This shows a slight prepon- 
derance of stout over slender types, roughly 4:3. 
Nov. 4th (twenty-four hours approximately after infection). 
—Three flies were dissected and examined, and smears made 
from each. In all of them active trypanosomes were found 
easily in the black blood, when examined fresh, but a hasty 
examination of the red blood did not reveal any trypanosomes ; 
VoL. 52, PART 2.,—NEW SERIES. 18 
