ARCHERINA, GOLENKINIA AND BOTRYOCOCCUS. 429 
specimens stained with hematoxylin en masse for several 
hours that a central substance exists which shows a deep 
purple stain (see Pl. 25, fig. 14). I did not detect any 
characteristic nuclear structure in this in specimens clarified 
and mounted in the usual way, but I am not in a position to 
say that such might not have been present though it escaped 
my observation. 
The cells appear to divide by binary fission along the long 
axis (figs. 15, 17). After fission the resulting cell-units 
separate and recede to a distance from one another equal to 
the short diameter of the cell, the interspace being filled by 
jelly (fig. 18). 
It is a long time since I had the opportunity of observing 
this organism, and I should urge those who may meet with it 
in the Lake district or elsewhere to direct their attention to 
the following points: 
(a) The nature of the green colouring matter. 
(b) The relation of the variable amount of yellow and red 
oily pigment to the season. 
(c) The mode of passage of the colouring matter into the 
jelly. 
(d) The existence of specimens showing colourless and of 
others showing green-coloured jelly. 
(e) The nuclear structure. 
(f) The possible occurrence of other modes of reproduction 
than the longitudinal fission leading to increase in the size of 
colonies. 
It does not appear that more than one European species of 
Botryococeus can be distinguished. The genus Ineffigiata of 
Mr. West is, | am informed by him, probably based upon a 
local growth variety of Botryococeus Braunii., 
May 31st, 1908. 
VOL. 02, PART 3.—NEW SERIES. 33 
