— 64 — 



Two days later the point at which the inoculation had been made showed an 

 exudation of liquid to such an extent as to leave no doubt of the disease produc- 

 ing power of the culture* 



One week later the plant showed signs of having the disease badly ; the stem 

 supporting the leaves was almost fallen away from the root, needing only the 

 slightest pull to bring that about. The leaves had withered and the plant had a 

 dying appearance. 



6.— Turnip Organism from Potato agar at 37" C x 520. 



On being cut up the turnip had the typical appearance of the original dis- 

 ease; from the crown downwards to half the depth of the root was completely 

 rotted, except that the shell of the root remained sound. 



Microscopic examination of the diseased portion showed the organism in 

 large numbers and evidently pure. Plate cultures were made from the diseased 

 part of the root and the organism re-isolated was in all respects identical with 

 the one originally isolated. 



In a second series of inoculations the root itself was inoculated with organ- 

 isms from a young agar culture by means of a needle puncture. 



After the same interval had elapsed as noted above an examination was made. 

 The appearance of the top part of the plant differed very much from the appearance 

 noted in trial I. The leaves appeared quite normal and, to the eye, the root also 

 seemed to be quite sound. On the root being cut open, the whole of the centre of 

 the turnip was rotted, the disease having in every respect the t}TDical characteristics. 



Both microscopic examination and replating on gelatine plates and other 

 media, yielded results which proved beyond doubt that the artificially produced 

 disease was identical with the original. 



It is of interest to note at this point that turnip may be badly diseased and 

 yet the appearance of the leaves and stem be such, that any other than a careful 



