— 66 — 



puncture in the crown, and while in 12 days time it looked apparently healthy, 

 sufficient evidence had already accumulated to show that the condition of the 

 leaves may not necessarily be a guide as to the soundness of the root. 



When this photograph had been taken .the turnip was cut in two and the 

 rotted condition as shown by Fig. 14 was made evident. 



Replating on gelatin from these rotted turnips in\ariably yielded an organism 

 identical in all respects with the original culture. 



In Fig. 10 is a photograph of a healthy cau]itl(jwer which was subsequent!} 

 inoculated bv needle puncture in the heart and in the stem with the turnip organ- 

 ism. 



8. — Turnip plaut inoculated in crowu with culture 

 isolated from diseased turuip produced in laboratory. 

 5 days after inoculation. 



Ill 14 days time the heart of the vegetable had rotted to a considerable ex- 

 tern, while the efifect on the stem had been such that several leaves had died ant! 

 fallen off, as shown in Fig. 1 1 . 



While we anticipate a continuance uf these inoculaiitm trials in gruvving 

 (jlants, as the different varieties are available in season, we recognise that the 

 ])ractic'il application centres abound the eft'ect on the soil after a crijp of turnips 

 infecte;! with this disease has been disposed of. 



In view of this, as a preliminary trial, we j)repared a series of pots with soil, 

 and steri'ized them iii sieam under pre>suic for three houis. A strong culture of 

 the organism was grown in beef bouillon, and, the maximum growth having been 

 obtained, we mixed with the culture a proportionate amount of a synthetic med- 

 ium — Uschinskvs. 



