—70 — 



Banana. In 24 liowrs growth and softening: in 48 hours pieces of banana 

 3-4 inch thick completely rotted with a waxy appearance and formation of gas. 

 Rhubarb. AVhitish growth on surface, but no softening in 24 hours. 

 Asparagus. Brown stain on all cut surfaces, no softening in 24 hours. 

 The media used in the cultural work consisted of — 

 Beef peptone gelatine. 



agar. 

 Potato gelatine, and 

 ■' agar. 



12.— Diseaseti Turnip Plant obtained direct from field. 



In preparing the two former, the standard methods of the American Society 

 of Bacteriologists were adhered to, the reaction to phenolphthalein being adjusted 

 to 1.5 p.c. acidity. 



To obtain the latter, healthy sound potatoes were grated and the juice thus 

 obtained strained through fine cloth and at once heated for half an hour in the 

 steamer at ioo°C. After standing for a few hours a comparativeily clear liquid 

 was obtainable. This poured off constituted with the addition of 5 p.c. of Na- 

 tirstofif Heyden and 12.5 p.c. gelatin or 1,5 p.c. agar the potato media. 



