256 THE UNIVERSITY SCIENCE BULLETIN. 



7. It ultimately seemed to prefer the streptococcus (chains of 

 diplococci) morphology. 



8. Complement fixation tests showed no normal complement 

 fixing bodies, in the normal rabbits used, for any of the pleomor- 

 phic streptococci, Streptococcus pyogenes or B. diphtheria. 



9. Complement fixation with respective immune sera showed 

 no relationship between the pleomorphic streptococcus and B. 

 diphtheria. This, however, does not necessarily prove no re- 

 lationship. 



10. While attempts to control the pleomorphism were largely 

 negative, I have some results which suggest that the salts which 

 crystallized out on the agar might be a factor in producing involu- 

 tion forms on the desiccated agar. 



11. I feel that a healthy skepticism is well worth while in all 

 work involving mutation, since errors in technique can lead to 

 very erroneous conclusions. The work of Mellon seems to be 

 thoroughly and carefully done and is worthy of consideration. 

 His results, even more than mine, point to an intermediate group 

 between the streptococci and diphtheria. 



BIBLIOGRAPHY. 



1. Gordon. 1901. Supplement to the Twenty-ninth Annual Report of 



the Local Governing Board, containing report of the medical officer, 

 1899-1900, p. 385. 



2. KuRTH. Arbeiten aus dem Kaiserlichen Gesundheitsamte, 1891, VII, 



p. 389. 



3. WiNSLOW. The Systematic Relationship of the Coccocese. 1908, p. 139. 



Wiley & Sons. 



4. Newman. Bact. and Pub. Health. 



5. Jean Broadhurst. J. of Inf. Dis., vol. 17, No. 2, 1915, pp. 277-330. 



6. Behring. Centralbld f. Bakt., 1892, XII, p. 192. 



7. Andrew and Horder. Lancet, 1906, II, p. 708. 



8. Besson. Practical Bact., Microbiology and Serum Therapy. Longmans, 



Green & Co., 1913, pp. 626-630. 



9. Winslow. The Systematic Relation of the Coccocese. pp. 166. 



10. Heineman. J. Inf. Dis., 1906, No. 3, p. 173. 



11. RosENOW. J. of Inf. Dis., 1914, vol. 14, No. 1. 



12. Billings and Rosenow. J. A. M. A., 1913, vol. 61, p. 2122. 



13. Vaughn. J. of Lab. and Clin. Med., vol. 1, 1915, No. 2, pp. 145-149. 



14. Broadhurst. J. of Inf. Dis., vol. 17, 1915, No. 2, pp. 277-330. 



15. NuTTALL AND Graham-Smith. The Bact. of Diphtheria. Cambridge 



University Press, 1913. 



16. Graham-Smith. Ibid., pp. 132-133. 



17. Graham-Smith. Ibid., pp. 158, 159, 160. 



