ALKALOIDS OP PUKATEA BARK. 125 



C. Preparation. — liecrvstallised from alcohol. Melting ponit 



200° C— 

 0'1259 gram, gave 0".'i319 gram. COo and 00676 gram. HgO. 

 7r89%carbon ' 5'97% hydrogen. 



D. Preparation. — Recrystallised from water — 



0'0776 gram, gave 0' 20545 gram. CO, and 0"0428 gram. H2O. 

 72'2% carbon 6'12% hydrogen. 



E. Preparation. — Recrystallised from absolute alcohol — 

 0'2558 gram, gave 10'8 cc. nitrogen at 764 mm. and 22° C. 



whence 4 '93% nitrogen. 



F. Preparation. — 



0'21S1 gram, gave 9'6 cc. nitrogen at 769 mm. and 20° C. 

 whence 5'2% nitrogen. 



Ci7Hi7N03 requires — 



Carbon ... 72"08 per cent. 



Hydrogen ... ... ... 6"01 „ 



Nitrogen ... ... ... 4'95 „ 



Oxygen ... ... ... ... 1696 „ 



lOO'OO per cent. 



Molecular Weight Determinations. 

 Calculated for Ci7Hij-N03. M. equals 283. 



0'1604 gram, depressed the melting point of 7' 12 grams, phenol 

 0-6°. M. equals 277. 



0'656 gram, depressed the melting point of 16'5 grams, phenol 

 0-925°. M. equals 303. 



It was found that pukateine behaves anomalously with pyridine, 

 ill which the alkaloid is very soluble.. In the experiments performed 

 v.'ith this solvent (B.P. 117°) to determine the molecvdar weight by the 

 boiling-point method — 



0-6188 gram, raised the B.P. of 11*05 grams, pyridine 0-05°. 



0'54 gram, raised the B.P. of 7 '4 grams, pyridine 0"05°. 



The research, which had been discontinued for want of material, 

 vs"as resumed on receipt of a supply of the bark collected in August, 

 1907, from trees in the Marlborough Sounds. 



A quantity of alkaloid has been obtained which has enabled me 

 to supply Professor Malcolm with one of its compounds. His pre- 

 liminaiy report is appended. 



In the following experiments the method of extracting jmkateine 

 (CiyHj^NOa) from the bark has been modified. It was found that 

 ciiloroform will extract the pukateine acetate from acid solutions. 

 iJark *fresh from the trees, weighing 166 lb., was thoroughly reduced 

 to a fine pulp in a heavy edge runner mill, and steeped for seven days 

 ill methylated spirit containing '5 per cent, acetic acid. The alcoholic 

 solution was pi'essed off and the treatinent repeated three times at the 

 same interval. By this means 21 '75 gallons of solution were obtainecl. 

 The alcohol was distilled off and the residue taken up with hot water, 

 cooled and filtered. The filtrate was shaken with, choloroform which 

 e.Ktracted the pukateine acetate. On distilling off the choloroform 



