324 H. B. FANTIIAM. 



12. ZiEMANN, H., 1S9S. — " Neue Uiitersuchungeii iiber die Malaria und 



den Malariaerregeni nahcsteliende Blutpaiasitcn," 'Deutsche med. 

 Wochensclir.,' xxiv, pp. 123-125 (especially p. 125). 



References quoted in Addendum. 



13. Christophers, S. R., 1907. — "Preliminary Note on the Development 



of Piroplasma canis in the Tick," 'Brit. Med. Journ.,' No. 2i02, 

 Jan. 12th, 1907, pp. 76-78, with 26 text-flgs. 



14. KiNOSiiiTA, K., 1907. — " Untersuclumgen iiber Babesia canis (Plana 



und Galli-Valerio)," 'Arch. f. Protistenkuude,' viii, pp. 294-320, tafs. 

 12 and 13. 



IX. EXPLANATION OF PLATE 18. 



Illustrating Mr. H. B. Fantliam's paper, " On the Chromatin 

 Masses of Piroplasma bigeminum (Babesia bovis), 

 the Parasite of Texas Cattle-Fever.^^ 



Figs. 1-7. — Lithograph drawings made from photo-micrographs of Piro- 

 plasma bigemin nm (Babesia bovis) from heart-smear, showing chromatin 

 masses and their distribution. These photo-micrographs were taken under 

 monochromatic (yellowish-green) light, using a Thorpe's grating. Zeiss's 

 3mm. apochromatic homog. immers. objective was used, with compensating 

 oculars 8 or 12. 



The lithograph drawings are twice the size of the photographs. 



These minute intra-corpuscular parasites were very difficult to focus for , 

 photography in order to bring out detail successfully, and so the relative sizes 

 of the nuclear masses do not appear always precisely correct. 



The red blood-corpuscles were in all cases only very faintly stained. 



Fig. 1. — Shows bridge of chromatin between nucleus and blepharoplast in 

 parasite to left. Note also pair of parasites on right. 



Fig. 2. — Pair of large pear-shaped intra-corpuscular parasites, showing 

 nucleus and blepharoplast in each, and loose chromatin at apex of one. 

 Magnification greater than in other figures. 



Pig. 3. — Blood-corpuscles showing rather better than in other figures, one 

 pair of parasites distinct, 



Pxc. 4.— One parasite shows three chromatin masses, and chromatin bridge. 



Fig. 5. — One parasite, on right, shows chromatin bridge faintly. 



Fig. 6.— Several pairs of parasites, on left, show nuclear dimorphism some- 

 what faintly. 



Fig. 7.— Parasites at top left-hand part of field show chromatin masses. 



