580 PROCEEDINGS OF SECTION G2. 



staining the contents. The lymphatic glands are more or less 

 Swollen, cedematous, and often hcemorrhagic. 



The pleural cavity generally contains more or less clear semi- 

 gelatinous effusion, which may be present even when there is no 

 peritoneal fluid, or may be practically absent when there is a 

 considerable quantity of peritoneal effusion. 



The lungs are often very oedematous, the interlobular tissue 

 being much distended by clear serosity, and in the sub- 

 pleural connective tissue small haemorrhages are frequently present. 

 Petechiae are common in the sub-epicardium and sub-endocardium; 

 they may be present in one and not the other, and may be absent 

 from both. 



Attempts to transmit the disease by feeding experiments 

 with virulent cultures have so far been negative. 



Cattle. — Only one animal has been inoculated — a three- 

 months' old calf in good condition — 0.5 cc. broth culture virulent 

 for guineapigs and sheep being injected subcutaneously. Beyond 

 a passing swelling the results were negative. 



Gttineapigs. — Guineapigs inoculated with small doses, 0.1 to 

 0.25 cc, of fluids containing the organisms or of pure cultures rapidly 

 succumb, generally within 20 hours of inoculation. Post-mortem 

 examinations show a large quantity of subcutaneous clear or 

 slightly blood-tinged effusion infiltrating the whole areolar tissue 

 of the limb, and often extending forward along the flood of the 

 abdomen to the shoulder. The muscles are usually haemorrhagic 

 at the point of inoculation, and there is more or less emphysema. 

 There may be no gas and no odour present, however, especially if 

 the muscles be little affected. Usually a small quantity of fluid 

 is present in the peritoneum. The spleen and kidneys are con- 

 gested. The liver, beyond congestion, rarely shows any change. 

 The stomach is often congested, and the small intestines are gene- 

 rally deeply congested, the mucosa infiltrated, and the lumen 

 frequently distended with clear, semi-gelatinous effusion. The 

 pleural cavit v rarely contains fluid, but the pericardium is frequently 

 distended with effusion. 



Rabbits. — Rabbits inoculated with 0.1 to 0.25 cc. of cultures 

 which have proved fatal within 20 hours to control guineapigs 

 remain normal, exhibiting no swelling or symptom of illness. With 

 0.5 cc, however, death results in less than 20 hours, the post- 

 mortem appearances being similar to those of guineapigs, with 

 the exception of bowel lesions, which are absent. 



Pigeons are readily affected, and succumb in less than 20 hours 

 with small doses of virulent cultures, the pathological changes 

 being confined to the muscles at the site of inoculation, which are 

 dark, swollen, and oedematous. 



Adult fowls at least are refractory to small doses. 



