" BRAXY " SHEEP DISEASE IN AUSTRALIA. 583 



lo ordinary doses of young serum broth cultures was not established, 

 for on being inoculated 19 days later, the sheep with 0.25 cc, the 

 ■guineapig with 0.15 cc. of such a culture, the former succumbed 

 in 60 hours, the latter in 20 hours. 



Another sheep (No. 28) inoculated with 5 cc. first sub-culture 

 in glucose serum broth six days old died in 40 hours, while a guinea- 

 pig (152) inoculated with 1 cc. died in 20 hours. 



These experiments did not give much prospect of using such 

 cultures as vaccines, although indicating a definite weakening. 



The Victorian Bacillus proved, however, more uniform and 

 gave better results. 



A sheep (No. 20) inoculated with 5 cc. eight days old of fourth 

 sub-culture in glucose serum broth remained normal. Twenty days 

 later it was inoculated with 0.25 cc. second sub-culture in serum 

 Tjroth two days old, and remained normal beyond passing lameness 

 and rise of temperature, whereas a control sheep (No. 25) inoculated 

 with a similar dose died in 38 hours. 



Another sheep (No. 31), inoculated with 3 cc. of an eight 

 days old third sub-culture in glucose serum broth, beyond a slight 

 ■swelling and passing lameness, remained normal. A guineapig 

 (149) inoculated with 1 cc. of the same culture also remained 

 normal. Twenty days later the sheep was inoculated with 0.25 cc. 

 24 hours old first culture in serum broth, and the guineapig with 

 •0.15 cc. The sheep developed no swelling but slight lameness and 

 definite rise of temperature for the first day, after which it resumed 

 normal. The guineapig died within 12 hours. 



Another sheep (No. 30) was inoculated with 5 cc. of a six days 

 old first sub-culture in glucose serum broth, and a guineapig (153) 

 with 1 cc. of the same culture. The guineapig died in 18 hours. 

 The sheep the following day was lame, the limb cedematous and the 

 temperature 104.2 ; but next day the animal was normal. Fifteen 

 days after this inoculation it received 0.25 cc. 24 hours old first 

 culture in serum broth from muscle, which the following day 

 induced lameness with slight swelling and some rise of temperature, 

 but complete recovery resulted 24 hours later. 



(For details, see Appendix A IV. and V., and Appendix B IV.) 



It is evident from these experiments, that by the use of cultures 

 on glucose media at least six days old one may hope to provide a 

 vaccine for at any rate the Victorian disease. 



Sheep immune to Victorian Bacillus, immune to Tasmanian 

 Bacillus. — That animals immune to the Victorian bacillus are also 

 immune to the Tasmanian bacillus is shown by the following : — 



Sheep (20, 30 and 31), which as shown were immunised against 

 virulent cultures of Victorian bacilli, proved when inoculated, 27, 

 19, and 15 days later respectively, with 0.25 cc. first culture of 

 Tasmanian bacillus, to be immune also to that inoculation, the only 

 abnormality shown being a sHght rise of temperature the following 

 day. These inoculations were controlled by injecting a control 

 sheep with a similar dose, and by inoculations of guineapigs, which 

 proved the virulence of the bacilli by the sheep dying in less than 

 48 hours, the guineapig in under 18 hours. 



