604 PROCEEDINGS OF SECTION G2. 



III. — -Experiments with Diseased Muscle dried at 55° C. 



Guineapigs 23 and 26. — Inoculated with fragment dried muscle, lamb 1, 

 mixed with broth. Death in I6and IShours. Post-mortem : .=ero-sanguineous 

 to clear effusion subcutaneously extending forward to axillfe : little peritoneal 

 fluid, sero-sanguineous effusion in pleura and pericardium ; liver, small 

 areas of necrosis in 23, but no necrosis in 26. 



Guineapig 25. — Inoculated with 0.05 cc. subcutaneous cedema of guinea- 

 pig 23. In two hours a swelling the size of a walnut was present and the 

 animal uneasy. Three hours later the swelling had increased, was hot and 

 painful. Six hours after inoculation the animal was very ill ; evidence of 

 great pain, squeaking convulsively, breathing rapidly. An hour later inter- 

 mittent muscular spasms appeared, head shaking ; does not move when 

 disturbed ; swelling now extending forward to elbow ; prostration followed, 

 and death occurred about 12 hours after inoculation. Post-mortem : much 

 subcutaneous effusion ; a little peritoneal and pericardial fluid ; liver con- 

 gested, and one or two patches of necrosis. 



Guineapig 38. — Inoculated with about 0-1 eg., of ground muscle as used 

 for sheep 3 and lamb 2. 24 hours later no swelling was observed. 12 hours 

 afterwards, however, there was a considerable swelling, and the animal 

 was prostrate. Death occurred about 36 hours after inoculation. Post 

 mortem : typical, but no liver lesions. 



Sheep 3 (5 years old). — Inoculated with 2 eg. dried muscle from lamb 1, 

 ground up and mixed with 2 cc. sterile broth. Twenty-four hours later 

 animal normal, with little swelling, and feeding well. Next day great lameness 

 observed, with swelling of whole limb. Sheep drowsy, uneasy, grinding teeth 

 intermittently, no appetite, temperature 100°. Illness rapidly became 

 aggravated, and death occurred 54 hours after inoculation. Post-mortem 

 examination : much subcutaneous oedema extending from foot to pubis and 

 several inches along abdomen. CEdema semi-gelatinous and clear, but for 

 faint blood tinge in thigh ; intermuscular tissue greatly infiltrated ; muscles 

 oedematous and pale, with little haemorrhage. No odour beyond a marked 

 muttony smell. Small quantity of clear effusion in pleural and peritoneal 

 cavities, and on exposure this rapidly became semi-gelatinous. Liver con- 

 gested with small sub-capsular areas of necrosis. Kidney congested ; spleen 

 normal ; abomasum — mucosa shows patches of intense congestion, some with 

 the surface necrosed with diphtheritic appearance, these areas varying in 

 diameter up to an inch. Submucosa not oedematous. Small intestines : 

 irregular areas of congested mucosa. Microscopical examination showed no 

 organisms present except in muscles and no oedema near region of inoculation. 



Lamb 4 (10 months old). — Inoculated with 2 eg. dried muscle of lamb 1, 

 ground up and mixed with 2 cc. of sterile broth. Twenty-four hours after 

 little swelling observed, animal feeding well. The following day animal 

 drowsy, no appetite, much swelling and lameness, but no. so intense as in 

 Sheep 3. Temperature 103°. Symptoms gradually became aggra^■ated 

 and death occurred during night, or about 60 hours after inoculation. 

 Post-mortem examination. — Subcutaneous tissues of inoculated limb 

 greatly infiltrated with straw-coloured oedema, which extends forward to 

 apex of sternum. At point of inoculation oedema blood-tinged and gas 

 present, with slight odour. Muscles pale and hemorrhagic in patches ; one 

 area of necrosis about two inches in diameter being present where needle 

 entered. Peritoneum : a pint of blood-tinged effusion ; liver congested, 

 no necrosis : kidneys congested ; spleen pulpy, but not enlarged ; sub- 

 lumbar muscles very oedematous. Abomasum : \ ery slight congestion ; 

 at pylorus small definite area deeply congested ; submucosa slightly oede- 

 matous ; duodenum congested for about the first two inches ; pleural cavity 

 about 10 oz. of straw-coloured effusion ; pericardium and heart normal. 

 Microscopical examination. — Many typical bacilli in oedema and muscles 

 in inoculated region ; subcutaneous cedema elsewhere sterile ; sub-lumbar 

 cedema typical bacilli. Peritoneal fluid : Typical bacilli, and long, thin 



