606 PROCEEDINGS OF SECTION G2. 



Lamb 5 (four months old, progeny of sheep 4, and had remained in same- 

 pen throughout). (See also guinea-pig 42.) Inoculated a month after sheep 

 4 with 0-5 cc. four days' old tenth subculture in ordinary serum broth from 

 lamb 1. Beyond a passing swelling the lamb remained normal. A month 

 after this inoculation, re-inoculated with 0-5 cc. 48 hours' old second sub- 

 culture from lamb 1. (See guineapig 52, inoculated with 0-1 cc. as control.) 

 Next day no change observed. Following day leg carried, with large oede- 

 matous swelling, dull, no appetite, temp., 105-8°. Condition gradually grew 

 worse, and death occurred in about 56 hours. Post-mortem examination 

 could not be made for about eight hours after death. Abdomen much dis- 

 turbed. Subcutaneous tissues all infiltrated with gas. Post-mortem dis- 

 colouration at thighs and elbows ; bloody froth from nostrils ; much oedema 

 infiltrating whole tissues of inoculated limb ; odour on incising skin marked, 

 but disappears rapidly ; little peritoneal effusion ; spleen pulpy ; kidneys, 

 softened and dark ; abomasum practically empty, mucosa congested, and 

 folds cedematous. Pleura : about 10 oz. deeply blood-stained fluid. Peri- 

 cardium distended with similar effusion. Lungs cedematous. Heart, 

 normal. 



Guineapig 37. — Inoculated with 0-1 cc. 30 days' old first subculture from 

 muscle lamb 3. (Culture only shows a few spores in deposit.) Beyond a 

 slight swelling, which disappeared next day, the animal remained normal. 



Guineapig 39. — Inoculated with O'l cc. one day old ninth subculture oa 

 ordinary serum broth carried on for two months. Death occurred n twc 

 days, during which time a large subcutaneous swelling had gradually 

 developed. Post-mortem : typical swelling of subcutaneous tissues,, 

 small quantity of peritoneal fluid ; no liver necrosis. (See sheep 4 

 inoculated with 0.5 cc. of same culture four days later.) 



Guineapig 115 and Rabbit 30 inoculated with 0-15 cc. first culture 30 

 days old. 



Guineapig 116 and Rabbit 31. — Inoculated with 0-15 cc, first 

 culture, being a culture derived from the fifth transfer of the same 

 portion of dried muscle as used to prepare culture for previous experi- 

 ment. This culture showed much gas formation, but no odour could be 

 detected. It was parallel to an ordinary fifth subculture, with the exception, 

 that the same portion of muscle was transferred each time, and the weakening 

 effect of each successive transfer would be on the bacteria within the muscle. 



Result of Inoculations. — Guineapig 115 and Rabbit 30 remained 

 normal. 



Guineapig 116. — 1st day: large typical swelling; 2nd day: swelling^, 

 smaller and firmer ; 3rd day : animal dull, swelling increased ; 4th day : 

 dead by daj'break, about 80 hours after inoculation. Post-mortem : 

 large subcutaneous cedematous swelling, extending forward to both, 

 axillae ; large quantity of clear peritoneal effusion, pinkish m colour, other- 

 wise little change. 



Rabbit 31. — In 24 hours a large subcutaneous cedematous swelling 

 present at region of inoculation. Death occurred about 36 hours after 

 inoculation. Post-mortem examination : much subcutaneous oedema, nO' 

 peritoneal fluid, liver affected with coccidiosis ; otherwise normal. 



Guineapig 115 and Rabbit 30 were re-inoculated 21 days after this- 

 experiment with 0-075 cc. first culture from sheep 1 muscle. The guineapig 

 died in less than 18 hours. Post-mortem examination showed typical sub- 

 cutaneous oedema, but otherwise little change. The rabbit died 46 hours, 

 after being inoculated, post-mortem examination showing typical subcu- 

 taneous oedema, and internal organs little altered. 



V. — Cultures in Glucose Serum Broth : Rapid Loss of Virulence.. 



Sheep 13, 3 years old. — Inoculated with 0-25 cc. 24 hours' old first 

 culture from muscle of lamb 1. Death occurred within 28 hours. Post- 

 mortem examination : typical subcutaneous oedema, no gas and no odour,. 



